加味生脉散对气虚型鼻咽癌大鼠的放射增敏作用及相关机制

Radiosensitization and Mechanisms of Jiawei Shengmai San on Nasopharyngeal Carcinoma in Rat with Qi Deficient Status

目的:探讨加味生脉散对气虚型鼻咽癌大鼠放疗增敏作用及相关机制。方法:SD大鼠于玻璃水缸中游泳,每天10 min,共30 d。同时在大鼠腋部皮下注射二亚硝基哌嗪(DNP),剂量为0.5 mg/只/次,每3 d注射1次,共注射30次,于90 d内完成,构建鼻咽癌大鼠模型。实验分为对照组、药物组、放疗组、药物+放疗组,通过测量肿瘤体积及计算抑瘤率,观察药物加味生脉散的放疗增敏作用;荧光定量PCR及Western-blot检测瘤组织多药耐药基因-1(MDR1)基因的表达差异。结果:肿瘤质量,对照组(1 230.0±106.8)mg,放疗组为(447.2±121.9)mg,与对照组比较,P<0.05,抑瘤率53.25%;药物加放疗组为(259.9±64.7)mg,与放疗组比较,P<0.05,抑瘤率72.81%;相对表达倍数,药物组为1.21,放疗组为1.85,药物加放疗组为1.34。结论:加味生脉散对鼻咽癌的放疗具有增敏作用,其增敏机制可能与抑制MDR1基因的表达相关。

Objective： To investigate radiosensitization and mechanism of Chinese medicine Jiawei Shengmai San on nasopharyngeal carcinoma. Method： The SD rats swimed in Glass tank for 10 minute in 30 days, at the same time, subcutaneous injection of dinitrosopiperazine （DNP） was performed, the dose was 0.5 mg every time, every 3 days the injection was carried out once for 90 days. Then, nasopharyngeal carcinoma models with Qi deficient status was produced. The samples were divided into control group, drug group, radiotherapy group, drug ＋ radiotherapy group. The radiosensitization effect was measured by measuring tumor volume and weight, and multidrug resistance 1 gene （MDR1） expression was detected by quantitative PCR and western-blot. Result： In the control group tumor weight was （1 230.0 ± 106.8） mg, in the radiotherapy group being （447.2 ± 121.9） mg. Compared with the control group, the ratio of tumor inhibition was 53.25% , there was a significant difference （P 〈 0.05）. In the drug ＋ radiotherapy group tumor weight was （259.9± 64.7） mg, the ratio of tumor inhibition was 72.81% , there also was a significant difference （P 〈 0.05）. The Relative expression ratio, in the drug group was 1.21, the radiotherapy group was 1.85, drug ＋ radiotherapy group was 1.34. Conclusion： Jiawei Shengmai San can enhance the sensitivity of radiotherapy to the nasopharyngeal carcinoma, and its mechanisms may be related to regulating MDR1 gene expression.