摘要
目的:研究肉桂多酚改善HepG2细胞胰岛素抵抗的分子机制。方法:以HepG2细胞为模型,设立对照组和肉桂多酚实验组,肉桂多酚组设5,10,15 mg.L-13种不同质量浓度组。肉桂多酚作用细胞24 h后,运用实时定量聚合酶链反应(RT-PCR)技术,检测肉桂多酚对胰岛素抵抗HepG2细胞内葡萄糖转运蛋白2(GLUT2)、磷酸烯醇丙酮酸羧激酶(PEPCK)和葡萄糖-6-磷酸酶(G-6-Pase)基因表达的影响。结果:与对照组比较,肉桂多酚作用细胞24 h后,降低了GLUT2 mRNA的表达(P<0.05);肉桂多酚能够明显降低PEPCK,G-6-Pase mRNA的表达(P<0.05,P<0.01),且随着浓度的升高,肉桂多酚对PEPCK和G-6-Pase mRNA表达的抑制作用越明显。结论:肉桂多酚对HepG2胰岛素抵抗具有明显的改善作用,其机制可能与降低细胞内GLUT2,PEPCK和G-6-Pase mRNA的表达有关。
Objective： To study the molecular mechanism of cinnamon polyphenols on improvement of insulin resistance in HepG2 cells in vitro. Method： The HepG2 cells were used as cell model. Cells were incubated with cinnamon polyphenols of three different concentrations （5, 10, 15 mg ·L^-1）. Control cells were incubated without cinnamon polyphenols. After 24 h, the mRNA expression of porcine glucose transporter 2 （GLUT2） phosphoenolpyruvate carboxykinase （PEPCK） and glucose-6-phosphatase （G-6-Pase） related to insulin resistance was detected by Real-time PCR （RT-PCR）. Result： Compared with the control group, the RT-PCR showed the mRNA expression of GLUT2 decreased after being treated with the cinnamon polyphenols （P 〈 0.05） ; the cinnamon polyphenols significantly decreased the expression of PEPCK and G-6-Pase mRNA （P 〈 0.05, P 〈 0.01） , moreover, the cinnamon polyphenols inhibited the expression of PEPCK and G-6-Pase mRNA in a dose- dependent manner. Conclusion： Cinnamon polyphenols can significantly improve insulin-resistance of HepG2 cells and its mechanism is related to the inhibition of GLUT2, PEPCK, G-6-Pase gene expression.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第24期276-279,共4页
Chinese Journal of Experimental Traditional Medical Formulae
