忍冬叶总黄酮对四氯化碳致急性肝损伤小鼠的保护作用

Protective Effects of Total Flavonoids from Leaves of Lonicera japonica on CCl_4-induced Acute Liver Injury in Mice

目的:研究忍冬叶总黄酮(TFLLJ)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:将60只昆明种小鼠随机分为正常组、模型组、TFLLJ高、中、低剂量组(400,200,100 mg.kg-1)和联苯双酯组(150 mg.kg-1),除正常组和模型组外,其余各组小鼠每日ig给药1次,共10 d。末次给药1 h后,除正常组外其余各组ip 0.1%CCl4花生油溶液(0.01 mL.g-1)1次,造成急性肝损伤。测定血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)含量,计算肝脏指数(LI),测定肝组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,观察肝组织病理改变。结果:TFLLJ(400,200,100 mg.kg-1)剂量组可降低CCl4致小鼠肝损伤血清中的ALT和AST(P<0.01或P<0.05)。可降低小鼠肝组织中MDA和SOD活性(P<0.01或P<0.05)。TFLLJ各剂量组能明显改善肝组织的病理变化。结论:忍冬叶总黄酮对CCl4致小鼠急性肝损伤具有一定的保护作用,可能与抗脂质氧化有关。

Objective： To study the protective effect of total flavonoids from leaves of Lonicera Japonica （TFLLJ） on acute hepatic injury induced by carbon tetraehloride （CCl4 ） in mice. Method： Sixty mice were randomly divided into six groups： the normal group, the model group, high-dose TFLLJ group （400 mg·kg^-1） , middle-dose TFLLJ group （200 mg·kg^-1 ） and low-dose TFLLJ group （ 100 mg·kg^-1） , bifendate group （ 150 mg·kg^-1 ）. Except for normal group and model group, the other mice were orally administered corresponding drugs once per day for ten days. Then, the treated groups and model group were ip given 0. 1% CC14 （0. 01mg·kg^-1） once to produce acute liver injury on the tenth day. The liver injury was evaluated by the indexes of serum alanine aminotransferase （AST）, aspartate aminotransferase （ALT）, liver index （LI）, liver superoxidedismutase （SOD） and malondialdehyde （MDA）, and the morphological changes of liver tissue. Result： FLLJ （400, 200, 100 mg·kg^-1） could remarkably resist the increase of ALT and AST, decrease the MDA level and could improve SOD activity （P 〈 0.01 or P 〈 0.05 versus model group）. Furthermore, TFLLJ could ameliorate the hepatic pathological changes. Conclusion： TFLLJ have protective effects on acute hepatic injury induced by CCl4 in mice. It may be related to its anti-oxidative effect for lipids.