雷公藤多苷对糖尿病大鼠结缔组织生长因子异常表达及肾功能的影响

Study of TG in CTGF Abnormal Expression and the Effect in the Renal Cortex of DM Rats

目的:研究雷公藤多苷对实验性糖尿病大鼠结缔组织生长因子(CTGF)异常表达及肾功能指标的影响,探讨其保护肾功能的机制。方法:雄性SD大鼠随机分为正常对照组、模型组和雷公藤多苷治疗组(1.8 g.kg-1.d-1灌胃,1次/d,连续8周),链脲佐菌素(STZ)腹腔注射建立糖尿病大鼠模型,给药第4,8周末分别测定大鼠体重、血糖、血尿素氮(BUN)、血肌酐(Cr)及24 h尿蛋白定量;HE染色法观察大鼠肾脏病理学变化;免疫组化染色测定肾组织中CTGF蛋白表达。结果:与正常对照组比较,模型组血糖、BUN,Cr,24 h尿蛋白定量在第4,8周末显著增加(P<0.01),肾脏CTGF蛋白表达增加(P<0.01),雷公藤多苷干预后可显著抑制模型组的病理改变(P<0.05或P<0.01)。结论:CTGF表达与糖尿病肾病的进展有关,雷公藤多苷可能通过下调CTGF的表达,减少糖尿病大鼠尿蛋白排泄量,改善肾脏功能,延缓糖尿病肾病肾脏的损害。

Objective： To study the mechanism of tripterygium glycosides （TG） in protecting the kidney by observing the effection of TG in abnormal expression of connective tissue growth factor （CTGF） and some factors of kidney function in artificial diabetes rats. Method： Forty-five Male SD rats were randomly divided into normal control group, diabetic nephropathy （DN） and diabetic nephropathy group treated with tripterygium glycosides （TG） group, 1.8 g·kg^-1·d^-1ig,once a day, using 8 weeks） DN group and TG group rats were intraperitoneal injected with sacrificed. The rats were sacrificed after 4 and 8 weeks. The body weight, blood glucose, blood urea nitrogen （BUN）, creatinine （Cr） and 24 h urinary protein were measured in each rat. The pathology changing of the rat kidney was observed by HE staining. The expression of CTGF protein in kidney was measured by immunohistochemistry. Result： Compared with control group, the level of blood glucose, BUN, Cr and 24 h urinary protein were significantly increased in DN group （P 〈 0.01 ）. The expression of CTGF protein was increased in DN group compared with control group （P 〈 0.01 ）; After intervention with tripterygium glycosides, these indexes were all significantly declined except blood glucose （P 〈 0.05, P 〈 0.01 ）. Conclusion： The TG on reducing the urinary protein in DM rats and improving renal injury in diabetic nephropathy may related to the inhibition of renal CTGF expression.