高效液相色谱法测定全血中葛根素含量

Determination of puerarin in whole blood by HPLC

建立一种测定全血中葛根素浓度的HPLC方法。方法 :以Nova pakC18、4μm色谱柱为分离柱 ,以甲醇 水 (30∶70 )为流动相 ,采用HPLC法测定。结果 :全血中葛根素在浓度为 2～ 2 0 μg·ml-1范围内线性良好 (r =0 .9997) ,平均回收率为(97.1± 0 .8) % ,供试液日内RSD为 1.15 % ,日间RSD为 5 .2 7% ,最低检测浓度为 0 .1μg·ml-1。结论 :本文建立了一个可靠、简便的测定全血中葛根素的HPLC法。

OBJECTIVE:An HPLC method was established for determination of puerarin in whole blood.METHODS:A Nova-pak C 18、4 μm radial compression column was used,with a mobile phase of 30% methanol and 70% water.RESULTS:The linear range was 2～20 μg·ml -1(r= 0.999 7),the average recovery was ( 97.1± 0.8)%,The RSD of within-day was 1.15%,and between-day was 5.27%.The detection limit of concentration was 0.1 μg·ml -1.CONCLUSIONS:This method was simple and feasible for the determination of puerarin in whole blood.