氨肽酶N在胆固醇结石成因中作用的初步研究

Preliminary study on effect of aminopeptidase N on etiology of cholesterol gallstones

目的 观察胆固醇结石病人和无结石者之间胆囊胆汁中氨肽酶N(APN)活性的变化及熊去氧胆酸 (UDCA)对APN活性的影响。方法 选择胆结石病人 ,随机分成两组 ,服用UDCA组和非服药组 ,无结石行上腹部手术的病例作为对照组。测定胆囊胆汁中蛋白质、APN活性、胆固醇、磷脂、胆盐、胆固醇饱和指数 ,比较各组结果。结果 发现APN活性在胆固醇结石病人胆囊胆汁中含量较无结石者高 (1 2 37± 0 371vs0 895± 0 2 47) ,P <0 0 5 ,服用UDCA后胆汁中APN活性较不服用UDCA者降低 (0 711± 0 2 45vs 1 2 37± 0 371) ,P <0 0 5。结论 本实验显示胆固醇结石病人与无结石者之间存在着APN活性上的差别 ;UDCA能够在降低胆固醇饱和指数的同时 ,也降低APN活性。

Objective To observe the difference of aminopeptidase N (APN) activity in gallbladder bile between patients with cholesterol gallstones and those without them and the effect of ursodeoxycholic acid (UDCA) on APN activity. Methods APN activity in gallbladder bile was compared between patients with cholesterol gallstones and those without them. The change in APN activity was observed after UDCA therapy in patients with cholesterol gallstones. Results APN activity in gallbladder bile of patients with cholesterol gallstones was higher than that of non gallstone patients (1 237±0 371 vs 0 895±0 247, P<0 05) and it decreased significantly by UDCA therapy (0 711±0 245 vs 1 237±0 371,P<0 05). Conclusions There is difference in APN activity in gallbladder bile between patients with cholesterol gallstones and those without them. UDCA therapy not only decreases CSI(cholesterol saturation index) but also lowers APN activity in gallbladder bile of patients with cholesterol gallstones.