子宫内膜异位症患者腹腔液白细胞介素6、8及转化生长因子β_(1)的测定

Measurements of Interleukin-6,Interleukin-8 and Transforming Growth Factor-β_(1)Levels in Peritoneal Fluid of Patients with Endometriosis

目的 探讨腹腔液中细胞因子在子宫内膜异位症 (内异症 )发病中的作用。方法 采用酶联免疫吸附试验 ,检测 31例内异症患者 (Ⅰ、Ⅱ、Ⅲ期各 8例 ,Ⅳ期 7例 )、2 2例非内异症患者 (对照 )腹腔液中白细胞介素 6 (IL 6 )、白细胞介素 8(IL 8)及转化生长因子 β1(TGF β1)的含量 ;并对内异症患者腹腔液中细胞因子浓度与美国生育协会修正标准分期 (R AFS)及痛经评分进行相关性分析。结果 内异症患者腹腔液中IL 6及IL 8含量 [( 1.8± 0 .4)ng/L ,( 1.7± 0 .5 )ng/L]明显高于对照者 [( 1.2±0 .2 )ng/L ,( 1.4± 0 .3)ng/L ,P <0 .0 5 ];TGF β1含量两者比较 ,差异无显著性 (P >0 .0 5 )。IL 6与R AFS评分有显著相关性 (P <0 .0 5 )。 3个细胞因子与痛经评分均无相关性 (P >0 .0 5 )。结论 内异症患者腹腔液中异常水平的IL 6及IL

Objective To investigate the role of cytokines in peritoneal fluid on pathogenesis of endometriosis (EM). Methods Interleukin6 (IL6), interleukin8(IL8) and transforming growth factorbeta 1 (TGFβ 1) contents in peritoneal fluid (PF) of 31 cases with EM were detected by enzyme linked immunoabsorbent assay (ELISA) and compared with the counterparts of 22 cases without EM (controls). The correlation analyses between cytokine concentrations in peritoneal fluid of EM patients and the severity of EM or dysmenorrhea score were performed. Results The peritoneal fluid from patients with EM contained significantly greater amounts of IL6 [(1.8±0.4) ng/L] and IL8 [(1.7±0.5)ng/L] than those in controls [(1.2±0.2)ng/L and (1.4± 0.3) ng/L respectively,P<0.05]. However, in the amounts of TGFβ 1 there were no significant difference (P >0.05) between the two groups. The highest PF IL6 and IL8 concentrations were found in stageⅡ,Ⅲ and stagⅠ,Ⅱ EM respectively. A significant correlation between PF IL6 content and the severity of disease was noted but there were no evidences of a relationship between concentrations of IL8 and TGFβ 1 and the severity of EM as well as between concentrations of three cytokines and dysmenorrhea score. Conclusion Unusual levels of IL6 and IL8 in PF of EM patients partly account for imbalance of the immunologically dynamic environment in peritoneal cavity of EM patients.