重度妊娠高血压综合征患者胎盘组织中细胞凋亡及其调控基因的研究

Study on Apoptosis and its Related Genes in Pregnancy Induced Hypertension

目的 研究细胞凋亡及其调控基因在重度妊娠高血压综合征 (妊高征 )发生、发展中的作用。方法 对 40例正常晚孕妇女 (对照组 )和 40例重度妊高征患者 (观察组 )的胎盘组织进行分析。用DNA缺口原位末端标记 (TUNEL)技术检测细胞凋亡 ;免疫组织化学方法检测促进和抑制凋亡基因 (bax/bcl 2 )的表达。结果 对照组胎盘细胞滋养细胞、合体滋养细胞中凋亡指数分别为 ( 1.1±0 .9) %、( 41.8± 1.5 ) % ;bax阳性率分别为 ( 1.0± 0 .9) %、( 2 8.9± 9.7) % ;bcl 2阳性率分别为 ( 2 .2±0 .8) %、( 2 2 .9± 0 .7) % ,总bax/bcl 2为 0 .7～ 1.7。观察组胎盘细胞滋养细胞、合体滋养细胞的凋亡指数分别为 ( 4.3± 1.2 ) %、( 45 .3± 1.4) % ;bax阳性率分别是 ( 2 .2± 0 .8) %、( 42 .5± 11.7) % ;bcl 2阳性率分别是 ( 3 .2± 0 .8) %、( 2 3 .3± 7.8) % ;总bax/bcl 2为 1.0～ 3.2。即 :对照组胎盘中有一定量的细胞凋亡、bax、bcl 2表达 ,bax/bcl 2表达间呈平衡趋势 ;观察组中细胞凋亡明显增高 (P <0 .0 1) ,bax表达也明显增强 (P <0 .0 1) ,bcl 2表达仅呈增高趋势 ,但差异无显著性 (P >0 .0 5 ) ,bax/bcl 2比值明显增高(P <0 .0 1)。结论 细胞凋亡及其调节基因的表达间具有一致性 ;细胞凋亡、bax/bcl

Objective To investigate apoptosis and its related genes expression in serious pregnancy induced hypertension (SPIH). Methods 40 cases of normal term pregnancy(NTP) and 40cases of SPIH were enrolled in this study. Apoptosis was detected using DNA in situ terminal deoxynucleotidyl transferas (TDT)mediated deoxyuridine triphosphate (dUTP)biotin nick ending labeling (TUNEL); bax and bcl2 were detected by immunohistochemical staining (SP). Results In NTP group apoptosis index(AI) of cytotrophoblasts(CT) and syncytiophoblasts(ST) in placenta was (1.1±0.9)% and (41.8±1.5)%;bax positive rate was (1.0±0.9)% and (28.9±9.7)%;bcl2 was (2.2±0.8)% and (22.9±0.7)% respectively;bax/bcl2 was 0.7～1.7.In SPIH group,AI was (4.3±1.2)% and (45.3%±1.4)%;bax was (2.2±0.8)% and (42.5±11.7)%;bcl2 was (3.2±0.8)% and (23.3±7.8)%;bax/bcl2 was 1.0～3.2. The difference between SPIH and NTP was significant respectively (P<0.01);bax/bcl2 was balance in NTP,while the balance was broken in SPIH. Conclusions Apoptosis and its related genes expression are simultaneous. The balance and imbalance of apoptosis and bax/bcl2 affect the structure and the function of placenta, and then may further affect the outcome of pregnancy.