子宫内膜癌组织碱性成纤维细胞生长因子表达和微血管密度的临床研究

Expression of Basic Fibroblastic Growth Factor and Microvessel Density in Endometrial Carcinoma

目的 探讨子宫内膜癌组织碱性成纤维细胞生长因子 (bFGF)表达和微血管密度 (MVD)与临床病理参数和预后的关系。方法 采用免疫组织化学技术检测 6 6例子宫内膜癌组织中bFGF的表达 ,利用Ⅷ因子相关抗原标记血管内皮细胞 ,选择能代表肌层浸润深度的切片进行染色。结果 子宫内膜癌组织中bFGF表达与病理分级呈明显正相关 (P <0 .0 1) ;子宫内膜癌组织中MVD与年龄、病理分级和肌层浸润深度呈明显正相关 (P <0 .0 1) ;子宫内膜癌组织中MVD和bFGF表达呈明显正相关(P <0 .0 1) ;bFGF强阳性表达或MVD较高的患者预后差 (P均 <0 .0 1)。结论 bFGF是子宫内膜癌组织重要的血管生成因子 。

Objective To investigate the relation of basic fibroblastic growth factor (bFGF) and microvessel density (MVD) with endometrial carcinoma. Methods A total of 66 specimens of endometrial carcinoma were evaluated by immunohistochemical techique with polyclonal antibody against bFGF. The microvessels were highlight by immunohistologically staining to detect factor Ⅷ related antigen. The area of the deepest myometrial invasion was selected for staining. Ninteen specimens of uterine prolapse were used for control. Results Expression of bFGF was significantly correlated with tumor grade (P<0.01). MVD was positively correlated with age, tumor grade and myometrial invasion (P<0.01, respectively). MVD was significantly correlated with expression of bFGF (P<0.01).Patients with bFGF strong expression and high MVD(≥9.4) had worse prognosis (P<0.01, respectively). Conclusions bFGF plays important role in angiogenesis in endometrial carcinoma. Expression of bFGF and MVD could be used as prognostic factors for survival in endometrial carcinoma.