血府逐瘀汤对非酒精性脂肪性肝病大鼠肝脏LXR-α表达的影响

Effects of "Xuefu Zhuyu Decoction" on LXR-α expression in rats of nonalcoholic fatty liver disease

目的探讨血府逐瘀汤对非酒精性脂肪性肝病(NAFLD)大鼠肝脏X受体α(LXR-α)的影响。方法雄性SD大鼠,分为正常组10只,血府逐瘀汤组(治疗组)和模型组各20只。治疗组和模型组用高脂饲料喂养10周建立NAFLD模型。造模成功后,各组进行相应干预共6周。采用HE染色法观察大鼠肝组织病理变化,测定各组大鼠肝功能,并用荧光定量RT-PCR检测各组大鼠LXR-α基因表达。结果①与正常组比较,模型组血清ALT、TCH升高,差异有统计学意义(P<0.05)。与模型组比较,治疗组血清AST下降,差异有统计学意义(P<0.05)。血清TG、FFA各组间比较,差异无统计学意义(P>0.05)。②与正常组比较,模型组肝组织TG、TCH、FFA、LXR-αmRNA水平均显著升高,差异有统计学意义(P<0.01)。与模型组比较,治疗组肝组织LXR-αmRNA水平显著降低,差异有统计学意义(P<0.01);而TG、TCH、FFA差异无统计学意义(P<0.05)。结论血府逐瘀汤能降低NAFLD大鼠LXR-α的基因表达,降低肝脏损害。

Objective To observe the effects of ＂Xuefu Zhuyu Decoction＂ on liver X receptor α（LXR-α） in the rats of nonalcoholic fatty liver disease（NAFLD）.Methods Male Sprague-Dawley rats were randomly divided into model group,treatment group and normal group.The nonalcoholic fatty liver disease was established by feeding fat-rich diets for ten weeks.The treatment group was given ＂Xuefu Zhuyu Decoction＂,the model group and normal group were given distilled water for six weeks.The levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,triglyceride（TG）,total cholesterol（TCH）,free fatty acid（FFA） in serum and triglyceride,total cholesterol and free fatty acid in liver tissues were detected.The expressions of LXR-α mRNA in rats liver were measured by fluorescent quantitative reverse transcription-polymerase chain reaction.Results The level of AST in serum was lower in treatment group than in the model group（P0.05）;the expression of LXR-α mRNA was higher in model group than in normal group（P0.01）,while the expression of LXR-α mRNA was lower in treatment group than model group（P0.01）.Conclusion ＂Xuefu Zhuyu Decoction＂ is effective for nonalcoholic fatty liver disease by reducing the expression of LXR-α mRNA and the level of AST.