血性脑脊液对内皮细胞分泌ET和NO的影响

Effect of BCSF on the Secretion or Endothelin and Nitric Oxide in Endothelial Cells

目的：观察血性脑脊液对内皮细胞分泌内皮素（Ｅｎｄｏｔｈｅｌｉｎ，ＥＴ）和一氧化氮（Ｎｉｔｒｉｃ Ｏｘｙｄｅ，ＮＯ）的影响。方法：建立脐静脉内皮细胞体外培养的方法，用放射免疫方法测定ＥＴ含量，用硝酸还原酶法测定ＮＯ的代谢产物ＮＯ_２～－／ＮＯ＿３～－（ＮＯ＿ｘ～－）含量，并观察血性脑脊液的作用。结果：不同时间血性脑脊液均使内皮细胞ＥＴ分泌增加，ＮＯ分泌减少。结论：血性脑脊液可使体外培养的内皮细胞ＥＴ分泌增加，ＮＯ分泌减少。

Aim: To study the effect of bloody cerebrospinal fluid (BCSF) on the secretion of endothelin (ET) and nitric oxide (NO) from human endothelial cells. Methods: The endothelial cells cultures from human umbilical vein were estab- lished. Concentration of ET and metabolic production of NO,NO_2^-/NO_3^-(NO_x^-) were determined by using radioimmunoassay and HNO3 reductase respectively, and effects of bloody cerebrospinal fluid (BCSF) were conducted. Results: BCSF of various stage could induce increase of ET and decrease of NO_x^- (P<0. 01) secreted by endothelial cells (P<0. 01). Conclusion: BSCF could induce increase of ET and decrease of NO_x^- secreted by endothelial cells cultured.