摘要
目的:观察血性脑脊液对内皮细胞分泌内皮素(Endothelin,ET)和一氧化氮(Nitric Oxyde,NO)的影响。方法:建立脐静脉内皮细胞体外培养的方法,用放射免疫方法测定ET含量,用硝酸还原酶法测定NO的代谢产物NO_2~-/NO_3~-(NO_x~-)含量,并观察血性脑脊液的作用。结果:不同时间血性脑脊液均使内皮细胞ET分泌增加,NO分泌减少。结论:血性脑脊液可使体外培养的内皮细胞ET分泌增加,NO分泌减少。
Aim: To study the effect of bloody cerebrospinal fluid (BCSF) on the secretion of endothelin (ET) and nitric oxide (NO) from human endothelial cells. Methods: The endothelial cells cultures from human umbilical vein were estab- lished. Concentration of ET and metabolic production of NO,NO_2^-/NO_3^-(NO_x^-) were determined by using radioimmunoassay and HNO3 reductase respectively, and effects of bloody cerebrospinal fluid (BCSF) were conducted. Results: BCSF of various stage could induce increase of ET and decrease of NO_x^- (P<0. 01) secreted by endothelial cells (P<0. 01). Conclusion: BSCF could induce increase of ET and decrease of NO_x^- secreted by endothelial cells cultured.
出处
《中国临床神经科学》
2000年第2期89-91,共3页
Chinese Journal of Clinical Neurosciences
关键词
蛛网膜下腔出血
血性脑脊液
内皮素
一氧化氮
subarachnoid hemorrhage bloody cerebrospinal fluid endothelin nitric oxide