拟南芥DWF4基因的内含子影响自身表达

Introns of DWF4 gene influence gene expression in Arabidopsis

为了进一步确定DWF4基因的内含子是否影响自身表达,构建了35S启动子驱动的带内含子和不带内含子的DWF4基因表达载体,分别转入拟南芥DWF4基因的弱突变体psc1。对T2代幼苗进行表型分析发现,转入带内含子的DWF4基因的转基因株系TgD4能完全恢复突变体的表型,而转入不带内含子的DWF4基因的转基因株系TcD4只能部分恢复突变体的表型。RT–PCR分析显示,TgD4的DWF4表达水平明显增加,说明内含子对DWF4基因的表达有增强作用。

To better understand the expression patterns of DWF4, expression plasmids with 35S CaMV constitutive promoter containing DWF4 gene with or without its own introns were constructed and transformed respectively into the pscl mutant deficient in the biosynthesis of brassinosteroids （BRs）. Phenotypic analysis showed that the transgenic lines TgD4 with the introns of DWF4 gene could rescue the defect of BR biosynthesis in pscl totally, whereas the transgenic lines TcD4 without the introns of DWF4 gene could only rescue the defect of BR biosynthesis partially. RT-PCR demonstrated that the expression of DWF4 in the transgenic expression ofDWF4 gene could be enhanced by its own introns. lines TgD4 was obviously increased indicating the