雌激素类似药NH1996对去卵巢大鼠海马突触素和钙结合蛋白表达的影响

The effects of estrogen and NH 1996 on synaptophysin, parvalbumin and calbindin G 28 K immunoreactivity in hippocampus of ovariectimized rats

采用大鼠卵巢切除 ( OVX)动物模型 ,用免疫组织化学方法结合图象分析 ,探讨雌激素、NH 1996对大鼠海马突触素及钙结合蛋白 ( Ca BP及 PV)的调节作用。图像分析表明 OVX组海马 CA1区及齿状回突触素、PV、Ca BP平均光密度及面积密度均显著低于对照组 ( P<0 .0 1) ,补充雌激素和 NH19963 5 d,这三项指标的平均光密度及面积密度均恢复至正常水平 ,与 OVX组相比 ,有显著性差异 ( P<0 .0 1)。推测雌激素、NH1996可能通过调节海马、齿状回神经元突触密度 ,改善学习记忆功能 ,缓解老年性痴呆病人的症状 ,并通过维持胞内 Ca2 + 的恒定达到保护神经元的目的。

Objectives:To observe the effects of estrogen and NH1996 on synaptophysin (syn), parvalbumin (PV) and calbindin D28K (CaBP) immunoreactivity in hippocampus of overiectomized (OVX) rats. Methods: An animal model was developed using OVX rats, and immunoreactive technique was used with the help of an image analysis system. Results: OVX rats resulted a decline in both integral optical density and area density of syn, PV and CaBP immunoreactivity in hippocampus CA1 and dentate gyrus. These effects can be reversed by estrogen and NH1996 given at 200 μg per rat, im, once every three days, for five weeks. Conclusions: The increase of the expression of the synaptic protein and neuronal calcium binding capacity might be related to both the improvement of memory/learning behavior and the maintainance of neuronal calcium homeostasis.