人结直肠癌组织CDK4基因和蛋白表达及其临床意义

The transcription of CDK4 gene and its protein expressions in human colorectal cacrcinoma and their clinical significance

目的 探讨人结直肠癌组织CDK4mRNA转录和蛋白表达水平及其临床意义。方法 通过Northernblot和Westernblot方法 ,分析 30例人结直肠癌和其相应正常粘膜组织中 ,CDK4基因mRNA转录和蛋白表达水平。结果 40 %的结直肠癌组织中CDK4mRNA转录水平增高 ,5 3 3%的癌组织蛋白表达水平增高。其表达情况与肿瘤分化程度有关 ,表达水平越高 ,分化程度越低 ,二者比较差异有显著性 (χ2 =13 0 5 ,P =0 0 2 3;χ2 =13 89,P =0 0 0 9)。结论 CDK4可能是结直肠癌的原癌基因 ,在肿瘤发生过程中起一定作用。可做为结肠癌基因治疗的理论依据。

Objective This study was conducted to investigate the levels of CDK4 mRNA transcription and protein expression of human colorectal cancers, and results were compared with the clinical pathological characteristics of the tumor. Method In 30 human colorectal carcinomas and corresponding adjacent normal mucosa, the levels of CDK4 gene mRNA transcription and protein expression were analyzed by means of Northern blotting and Western blotting, respectively.Results The overexpressions of CDK4 transcription and protein level were 40% and 53 3%, respectively, and negatively correlated with the differentiation of the tumor (χ 2=13 05, P=0 023; χ 2=13 89, P=0 009).Conclusion CDK4 gene may play a role in tumorigenesis in human colorectal carcinomas. This result may provide a theoretical evidence in the gene therapy of colon cancer.