半支莲主要化学成分的HPLC-TOF/MS分析

Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS

目的通过高效液相-飞行时间质谱(HPLC-TOF/MS)联用技术,对中药半枝莲的主要化学成分进行有效的鉴定。方法色谱分离采用SHISEIDO CAPCELL PAK C18柱(100 mm×3.0 mm,3μm),流动相分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.6 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。结果在优化的液质联用条件下,通过飞行时间质谱鉴定出半枝莲中15个成分。结论通过HPLC-TOF/MS联用技术,为鉴定半枝莲中的化学成分建立起了一种快速、高效的分析方法。

Objective To analyze chemical constituents of Scutellaria barbata D. Don by high pressure liquid chromatography- time of flight mass spectrometry （HPLC-TOF/MS）. Methods A SHISEIDO CAPCELL PAK C18 （100 mm × 3.0 mm, 3 μm） was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 15 major constituents in Scutellaria barbara D. Don were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of Scutellaria barbara D. Don.