HPLC法测定中药山楂中有效成分绿原酸的含量

Determinatin of chlorogenic acid in hawthorn by HPLC

目的建立测定山楂中绿原酸含量的高效液相色谱(HPLC)法。方法色谱柱为Sunfire C18柱(250 mm×4.6mm,5μm),流动相为甲醇-0.1 mol/L磷酸二氢钠缓冲液(25∶75),流速为1.0 ml/min,柱温为28℃,检测器波长为327 nm。结果绿原酸的质量浓度在7.03～70.3μg/ml范围内与峰面积值线性关系良好(r2=0.999 6),精密度RSD为0.27%,平均加样回收率为96.92%,RSD为0.92%(n=6)。结论 HPLC法简便易行、结果可靠,可以快速、准确地测定山楂中绿原酸的含量。

Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire CIS （4.6 mm × 250 mm,5 μm） column was used. The mobile phase was methanol： sodium dihydrogen phosphate（25 ： 75）. The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03 - 70.3μg/ml （ r = 0. 999 6）, RSD was 0. 92% （ n = 6） and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.