肿柄菊粗提物对果蝇胃毒、驱避、触杀、熏蒸效果的研究

The Research of the Stomach,Repellent Contact and Funigation Effect on Drosophila from Different Tithonia diversifolia Extracts

为了寻找防治果蝇的新型植物源杀虫剂。采集肿柄菊地上部分,分别收集了石油醚、乙酸乙酯、乙醇的提取物,比较了不同极性溶剂提取物对果蝇的胃毒、驱避、触杀及熏蒸作用。结果表明:乙酸乙酯提取物在10mg/mL时对果蝇胃毒效果最好,72h可达93.33%;3种提取物在50mg/mL时对果蝇均有很强的驱避效果,乙酸乙酯和乙醇提取物在8h可达100%,石油醚次之,可达85%;乙酸乙酯及乙醇提取物在50mg/mL时对果蝇熏蒸效果最好,120min可达100%;3种提取物对果蝇的触杀效果均较低。因此,肿柄菊次生代谢产物具有一定绿色农药的开发潜力。

In order to find a new plant pesticide, the petroleum ether extract, ethyl acetate and ethanol extracts from aerial parts of Tithonia diversifolia were isolated. The stomach action, repellent action, contact action, fumigation action of different Tithonia diverslTolia extracts were studied and compared with drosophila as test object. The results showed that： the ethyl acetate extracts had very strong stomach effect on drosophila in the 10 mg/mL concentration and the stomach rate could reached 93.33% at 72 h. In the 50 mg/mL concentration, the three extracts had very strong repellent effect, the ethyl acetate and ethanol extracts could reached 100%, the petroleum ether extract could reached 85%. The ethyl acetate and ethanol extracts had stronger fumigation effect than the petroleum ether extract and both of them can reached 100% at 120 min. The contact action on drosophila of three extracts were all lower. Therefore, the secondary metabolites of Tithonia diversifolia showed that it has some development potential.