亚胺培南/西司他丁对肺炎大鼠的免疫调节作用

Immunomodulating effects of imipenem/cilastatin in pneumonic rats

研究亚胺培南 /西司他丁 (IPM/CS)对肺炎大鼠的免疫调节作用。采用气管内注入感染法建立肺炎克雷伯氏菌肺炎模型 ,按临床治疗剂量折算出大鼠等效剂量 ,连续 7d腹腔注射 IPM/CS及阿米卡星(AMK) ,活体取材测定免疫指标。结果显示 IPM/CS使 T淋巴细胞 CD+ 3亚群及 CD+ 4 /CD+ 8比值升高 ,增强巨噬细胞吞噬功能 ,对巨噬细胞 ADCC活性及 ACP活性和中性粒细胞化学发光无明显影响 ;AMK则使 CD+ 3亚群及 CD+ 4 /CD+ 8降低 ,还降低巨噬细胞 ADCC活性和吞噬功能。提示 IPM/CS和 AMK分别促进和抑制了肺炎大鼠 T淋巴细胞和巨噬细胞功能。

To study the immunomodulating effects of imipenem/cilastatin (IPM/CS) and amikacin (AMK) in pneumonic rats. The male wistar rats were infected with Klebsiella pneumoniae species by the intratracheal peroral routs. IPM/CS and AMK were adiministered at therapeutic doses intraperitoneally for 7 days and several immunologic parameters were measured. IPM/CS enhanced the number of CD + 3 and CD + 4/CD + 8 value. The phygocytosis of macrophages was increased by IPM/CS. The weight of organs (thymus and spleen), B cell density in the germinal centre of splenic follicles, ADCC and ACP activity of the macrophages, PMN CL had no significant alteration in IPM/CS group. IPM/CS and AMK could promote or restrain the immune response of T lymphocytes and macrophages in pneumonic rats, respectively.