江苏省1996年～1999年急性弛缓性麻痹病例病原学研究

Etiological study of AFP from 1996~ 1999 in Jiangsu province

对江苏省1996年～1999年817例急性弛缓性麻痹（AFP）病例进行了检测，分离出病毒152株，阳性分离率为18．60％，其中脊髓灰质炎（简称脊灰）病毒40株，分离率为4．90％，用PCR－RFLP分析法鉴定其型内待征，均为疫苗株。分离到非脊灰肠道病毒（NPEV）112株，分离率为13．70％，不同月份分离率不同，以夏季为高。不同年龄组，不同采便时间、不同地区间NPEV分离率无显著性差异。NPEV分离率达到了世界卫生组织（WHO）推荐的非脊灰肠道病毒分离率标准（10％），AFP病例中存在3个血清型，全部为脊灰疫苗株，表明我省脊灰实验室监测系统是敏感的。

] This article reported the surveillance results of AFP cases in Jiangsu han 1996~ 1999. Totally,817 AFP fecal specimens were tested and 152 viruses derived, the virus positive isolation rate was 18.60% .40 of them were polio viruses, the positive rate was 4. 90 %, by using PCR - RFLP technique to analyze intratypic differentiation of the 40 isolated polio virus strains, it confirmed they were all polio vaccine virus strains. 112 of them were the non - polio enterovirus, the positive rate was 13. 70 %. A prominent seasonal peak of the non - polio enterovirus rate is occured in summer, and significant difference was found in the different month of feces collection. No significant difference was found the isolation rate of the non - polio enterovirus in terms of diffrent age, areas and days of fecal specimen gathering, though the difference of the non - polio enterovirus rate was existed. The non - polio enterovirus rate has reached the target of the non - poio enterovirus isolation rate(10% ) recommended by World Health Organization(WHO), and the viruses types of polio cases from 1996~1999 converted into three types, and all were vaccine strains. This showed the Lab surveillance system in Jiangsu was sensitive.