胚胎干细胞移植于内膜损伤小鼠的初步研究

Preliminary Study of Embryonic Stem Cells Transplanted into the Injuried Endometrium of Mouse

目的:了解胚胎干细胞是否能选择性定植于子宫内膜新损伤小鼠模型的子宫腔,并助其修复损伤。方法:制备新鲜子宫内膜损伤的小鼠模型,利用带增强型绿色荧光蛋白(enhanced green fluorescentprotein,EGFP)基因标记的小鼠胚胎干细胞(ESC)体外培养后移植至模型鼠宫腔,对比观察ESC在内膜损伤模型鼠宫腔内滞留与定植情况,及其对损伤子宫修复的影响,以及对干细胞移植后成瘤性的影响。结果:移入的ESC可以在内膜受损模型小鼠的宫腔内存活3周以上,且随着时间的推移,在宫腔内形成肿瘤的机会增加。静脉注射ESC后,可见ESC在内膜损伤宫腔及躯体其他损伤部位聚集,而不见于无损伤的对侧宫腔。结论:损伤是诱导干细胞募集的重要因素,如何诱导ESC定向分化及控制成瘤性仍有待进一步研究解决。

Objective： To investigate whether the embryonic stem cells （ESC） could selective implant into the uterine cavities of endometrium-injured mouse and help the endometrium reparation. Methods：The mouse model with freshly injured endometrium was established. ESCs derived from EGFP transgenic mouse were transplanted into the endometrium-injured mouse uterines. Then, the retention and the effects of ESCs on the reparation of injured endometrium and the tumorigenicity of post-transplanted stem cells were observed. Results：The ESCs survived in the uterine cavities more than 3 weeks with the rate of tumorigenicity increasing in time dependently. ESCs intravenous-injected could concentrated in the endometrium-injured uterine cavities and other injured parts of the body. Conclnsions：Injury is the most important inducing factor of ESCs recruitment. However,the problems of inducing directional differentiation and managing the neoplasia of ESCs are remain unsolved.