卵泡刺激素及戊酸雌二醇对小鼠卵母细胞体外成熟的影响

Effect of Follicle Stimulation Hormone and Pentanoic Acid Estradiol on Mouse Oocyte in Vitro Maturation

目的:探讨卵泡刺激素(FSH)、戊酸雌二醇(E2)对小鼠未成熟卵母细胞体外成熟、受精及卵裂能力的影响。方法:对雌性小鼠进行超促排卵后处死小鼠,取出卵巢,刺破卵泡,收集形态较好的未成熟卵母细胞,随机分为6组,在不添加激素的基础培养液中以及分别添加10,50 IU/mL的FSH及1,10,100 mg/L的戊酸E2的培养液中培养24 h后,观察各组卵母细胞的成熟情况。对已成熟的卵母细胞进行体外受精,观察卵母细胞受精情况。受精后继续培养48～72 h,观察其卵裂情况。比较各组的成熟率、受精率和卵裂率。结果:①各浓度FSH组及各浓度戊酸E2组与对照组相比,成熟率[生发卵泡破裂率(GVBD率)、MⅡ率]及卵裂率的差别均无统计学意义(P>0.05)。②10,50 IU/mL FSH组以及10 mg/L戊酸E2组的受精率分别为41.18%,40%和42.86%,均高于对照组(19.44%),差异有统计学意义(P<0.05);1,100 mg/L戊酸E2组与对照组相比受精率差异均无统计学意义(P>0.05)。③各浓度FSH组之间以及各浓度戊酸E2组之间成熟率(GVBD率、MⅡ率)、受精率及卵裂率的差别均无统计学意义(P>0.05)。结论:在小鼠体外成熟培养液中添加10,50 IU/mLFSH及10 mg/L戊酸E2能促进小鼠卵母细胞胞质的成熟,从而提高受精率。

Objective：To investigate the effect of Follicle Stimulation Hormone （FSH） and pentanoic acid estradiol on the mouse oocytes in vitro maturation, and the capacity of in vitro fertilization and merogenesis. Methods：The female Kuming mice were conducted superovulation. Immature oocytes were collected from those ovaries. Those immature oocytes were classified as six groups： matured for 24 h in the basal culture fluid without hormone as control; and the culture fluid added 10 and 50 IU/mL FSH; and the culture fluid added 1, 10, 100 mg/L pentanoic acid estradiol, The status of oocytematuration was observed, those matured oocytes were then inseminated in-vitro. The capability of fertilization was observed, and the inseminated oocytes were cultured for another 48-72 hours. The cleavage rate was compared at last. Results： （~） Compared with control, all treated groups had similar rates of undergoing GVBD, MII and cleavage （P〉0.05）. （~）The fertilization rates in those groups treated with 10, 50 IU/mL FSH and 10 mg/L pentanoic acid estradiol were 41.18% ,40% and 42.86% respectively. Those rates were all higher than that in control group（19.44%） （P〈O.05）. Compared with control, two groups treated with 1 and 100 mg/L pentanoic acid estradiot had similar cleavage rate and fertilization rate （P〉0.05）. （~）The percentages of GVBD, MII and cleavage in groups treated with FSH and pentanoic acid estradiol of different concentrations were similar （P〉0.05）. Conclusions：The addition of 10 and 50 IU/mL FSH or 10 mg/L pentanoic acid estradiol in the mouse IVM culture solution could promote the mature of mouse oocyte cytoplasm, and increase the fertilization rate.