紫外线对豚鼠郎格汉斯细胞功能影响的研究

STUDY OF THE EFFECTS OF UVB ON LC IMMUNE FUNCTION

目的 :探讨紫外线 (Ultraviolet)的免疫抑制机制。方法 :体外观察紫外线B(UVB)对豚鼠郎格汉斯细胞 (LC)刺激自体淋巴细胞增殖能力的影响。结果 :UVB以剂量依赖性方式抑制LC刺激自体淋巴细胞增殖能力。低剂量UVB( 2 5J/m2 )没有抑制作用 ,50J/m2 抑制率为 2 2 4 % ,10 0J/m2 抑制率为 54 5% ,2 0 0J/m2 几乎可以完全抑制淋巴细胞增殖反应。结论 :紫外线可以直接抑制LC的功能 。

Objective:To study the immunosuppression mechanism of Ultraviolet (UV) . Methods: In vitro,the auto lymphocyte proliferation test with LC in guinea-pig was investigated . Results: It showes that UVB significantly inhibited LC auto-stimulatory property in dose-dependent way. That enriched LC suspensions exposure to low doses of UVB(25 J/m 2) radiation does not significantly affect their auto-stimulatory capacity , the inhibition ratio of UVB irradiation is 22.4%,50% in dose of 50J/m 2 and 100J/m 2. At 200 J/m 2 it almost completely abrogated the lymphocyte proliferation.Conclusion: UVB can inhibit the function of LC directly. The mechanism of immunosuppression induced by UVB may be proposed that it may interrupt the auto-regulation of surface molecules of LC.