利用RT-PCR技术检测辣椒轻斑驳病毒的研究

Study on Detection of Pepper Mild Mottle Virus by RT-PCR

在田间病害调查过程中发现的一批感染病毒病的辣椒样本,经摩擦接种技术将病毒接种于烟草叶片,以期进行病毒鉴定。结果表明:经摩擦接种后烟草叶片显示斑驳、花叶症状;采用CTAB法提取辣椒及烟草叶片总RNA,利用RT-PCR技术,以辣椒轻斑驳病毒(Pepper mild mottle virus,PMMoV)特异引物,扩增出了预期大小片段,经测序证明其为PMMoV特异片段。

A number of peppers infected virus were found by field survey. The virus was inoculated mechanically to tobacco leaves,and the symptoms including mottle and mosaic leaves, could be observed in tobacco. Total RNA, extracted from infected pepper and tobacco with CTAB method,was used as template in RT-PCR with the special primers of pepper mild mottle virus （PMMoV）. Expected fragments were obtained and sequenced. It showed that this virus was identified as PMMoV. RT-PCR detection system for PMMoV was established.