香菇菌柄基部多糖的提取及其清除DPPH自由基作用的研究

Research on the Extraction of Polysaccharides from Stipe Base of Lentinus edode and Function of Scavenging of DPPH Radical by the Polysaccharides

以香菇菌柄基部和纤维素酶为试材,研究了纤维素酶解法提取香菇菌柄基部多糖的最佳工艺。结果表明:粉碎粒度200目,酶解温度35℃,酶解时间3h,香菇菌柄基部多糖的提取率可达到5.124%;初步纯化后的多糖对DPPH自由基有较强的清除活性,当清除率为50%时,香菇多糖的IC50为0.13mg/mL。

Taking the stipe base of Lentinus edode and cellulose as materials, the optimum extraction process of polysaecharides from stipe base of Lentinus edode were studied using cellulose method. The results showed that the optimum conditions for extraction process were as follows,the powder size of L. edode 200 mesh,extracting temperature 35℃ ,extracting time 3 h, and the extraction rate of polysaccharides could reach 5. 124%. The preliminarily purified polysaccharides had powerful scavenging efficiency to DPPH radical. Its IC50 was 0. 13 mg/mL.