宁南山区本氏针茅自然种群遗传分化的ISSR分析

ISSR analysis of genetic differentiation of Stipa bungeana populations in the mountain area of southern Ningxia

利用ISSR分子标记,分析宁南山区6个本氏针茅(Stipa bungeana)自然种群的遗传分化及其群体遗传结构,旨在探讨本氏针茅遗传变异产生的分子生态机理,为其资源保护提供理论依据。主要结果如下,在本氏针茅6个种群中,15条ISSR引物共扩增出244条可统计条带,其中多态性条带239条,多态性位点比率(PPB)为97.95%,Nei’s基因多样性指数(H)为0.254 4,Shannon信息指数(I)为0.396 6,各种群之间具有较高的遗传差异。分子方差分析(AMOVA)表明本氏针茅遗传变异的58.06%发生在种群内,41.94%的遗传变异发生在种群间。Mantel检测结果显示本氏针茅6个种群间的遗传距离和地理距离之间均无显著相关性。

In order to study and explore the mechanism of molecular ecology of Stipa bungeana, inter-simple sequence repeat （ISSR） marker was used to analyze the genetic differentiation and population structure of six natural populations of S. bungeana from the mountain area of southern Ningxia. The results showed that fifteen primers generated a total of 244 amplification bands from the six natural populations, of which 239 （97.95%） were polymorphic bands. Nei＇s gene diversity index and Shannon information index were 0. 254 4 and 0. 396 6, respectively, which indicated that the high genetic diversity was detected at the species level. AMOVA analysis revealed a high level of genetic variation within population （58.06 %） and a relative lower of genetic variation among populations （41. 94%）. There was no significant correlation between genetic distance and geographic distance by Mantel test.