维甲酸处理前后外胚间充质细胞中Cyclin D1蛋白表达的定量分析

The Quantitative Analysis of Cyclin D1 Expression in RA treated and ;non RA treated Ectomesenchymal Cells in vitro

目的 :探讨维甲酸对胎鼠面突外胚间充质细胞的作用和CyclinD1变化之间的关系。方法 :原代培养GD12 1 5(妊娠第 12天第 15小时 )胎鼠面突的外胚间充质细胞 ,原代培养细胞后 ,观察维甲酸处理前后细胞生长曲线的变化 ,采用免疫组织化学和图像分析仪对维甲酸处理后CyclinD1蛋白的表达变化进行定量分析。结果 :维甲酸处理后 ,外胚间充质细胞的生长受到抑制 ,正常的外胚间充质细胞中 ,CyclinD1的表达量较高 ,维甲酸处理后 ,其表达量显著降低。结论 :维甲酸所引起的细胞生长抑制与CyclinD1的表达降低之间密切相关 。

Objective: To elucidate the alteration of Cyclin D1 protein between RA treated and the non RA treated ectomesenchymal cells in embryonic mice. Methods: Ectomesenchymal cells in the developing orofacial processes were explanted from embryonic BALB c mice on generation day 12 + {15 } (the 15th hour of the 12th day). Primary cells were cultured using the tissue mass method. The variants of growth curves were examined in the RA treated and normal groups. Protein of Cyclin D1 was detected using immunohistochemistry. The positive degree of Cyclin D1 staining was analyzed using image analysis. Results: The findings showed that the growth of ectomesenchymal cells was inhibited dramatically. Cyclin D1 expressed strongly in normal ectomesenchymal cells. However, its expression decreased evidently after RA treated. There was significant difference between the test group and the normal group (P<0 01).Conclusion: Low expression of Cyclin D1 may contribute to the inhibition of ectomisenchymal cells. It is necessary for Cyclin D1 to express appropriately during the normal meiosis of ectomesinchymal cells. ;