两种水溶性甲壳素和丝素共混膜的结构与性能

Structure and Performance of Two Water-Soluble Chitin and SF Composite Membrane

制备了两种水溶性甲壳素:N-乙酰化壳聚糖(N-ACTS,DS=0.55)和6-O-羟乙基甲壳素(6-O-GC,DS=1.0),将其分别与丝素(SF)混合,制备了水溶性均相混合的复合膜;红外光谱(FT-IR)证明了均相混合的复合膜中两种天然高分子官能团结构的变化,相互之间形成了氢键结合,成为均一的混合体,表明天然高分子之间具有良好的相容性;原子间力显微镜(AFM)表面形貌观察表明:均相混合复合膜的表面比单一丝素蛋白质和多糖螺旋立体构造分布均匀表面光滑;体外细胞培养实验表明:人皮肤纤维芽细胞在两种复合膜上均生长良好,具有良好的生物相容性和医学应用前景。

Water-soluble homogeneous mixed composite membranes were prepared by mixing two water-soluble chitin, nitrogen acylation chitosan （ N-ACTS, DS = O. 55 ） and 6-O-hydroxyethyl chitin （ 6-O-GC, DS = 1.0） respectively with silk fibroin （SF）. The fourier transform infrared spectroscopy （FT-IR） demonstrated that the functional group structure on two kinds of natural polymer were changed,formed the hydrogen bond combination and a homogeneous mixture,indicated that the two kinds of natural polymer had good compatibility. The surface morphology of composite films were observed by the atomic force microscope （AFM） , the results showed that the surface of the homogeneous mixed composite films were more uniform distributed and glossy than pure SF protein and polysaccharose spiral three-dimensional structure,people skin fiber buds cells on the two kinds of mixture membranes were nice in vitro cell culture experiment,indicated that N-ACTS/SF and 6-O-GC/SF composite films had good biocompatibility and medical application prospect.