日粮铬对热应激獭兔肾脏组织结构及抗氧化功能的影响

Effect of adding chrome in diets on structure and oxidation prevention function in kidney tissue of rabbit under heat stress

为了研究三氯化铬对热应激獭兔肾脏结构及组织中抗氧化功能的影响,本试验选用3月龄健康獭兔72只,随机分为6组,每组12只,对照组饲喂基础日粮,分别在基础日粮中添加0.2、0.4、0.6、0.8、1.0 mg/kg铬量的三氯化铬,作为试验Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组,在热应激条件下(獭免饲养于人工气候仓内,气温设定为30～35℃,24 h循环变温,35℃维持4 h,)饲养30 d。显示,热应激条件下,在獭兔基础日粮中添加一定量的三氯化铬不仅能有效降低热应激对獭兔肾脏组织结构的损伤,还可显著提高肾脏组织中GSH-Px、SOD和CAT活性,降低肾脏组织中MDA含量(P<0.05或0.01)。结果提示,夏季高温环境下,在獭兔基础日粮中添加0.6～1.0 mg/kg铬量的三氯化铬可通过增强机体的抗氧化功能,抑制机体内自由基和脂质过氧化物的产生,保护机体肾脏组织结构完整,维持机体正常生理功能。

The design was used to study the effect of adding chrome in diets on structure and oxidation prevention in kidney tissue of rabbit under heat stress. Seventy two rabbits,3 month-old,were divided randomly into 6 groups. The control group was fed the basal diet, and the treatment groups were given the diet supplemented with 0.2,0.4, 0. 6, 0.8,1.0 mg/kg chrome, respectively. The rabbits were raised under heat stress （raised in artificial climate laboratory,with temperature 30-35℃, a cycling 24 hour, 35℃ for 4 hours）for 30 days. The results showed that the diet adding proper chrome not only can efficiently respite damage of on kidney tissue structure caused by heat stress, but also rises activity of GSH-Px.SOD and CAT,decreases content of MDA in kidney tissue （P〈0.05 or P〈0.01） ,indicating that in high temperature condition, the diet adding 0.6-1.0 mg/kg chrome could strengthen body oxidation prevention function, restraint ROS, protect kidney tissue structure integrity, support body normal physiologic function.