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促黄体素受体LHR的一种剪接体在牛黄体不同发育时期表达量差异的研究

Expression of luteinizing hormone receptor in different stages of bovine corpus luteum
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摘要 利用TaqMan-MGB探针实时荧光RT PCR检测方法,对牛黄体中促黄体素受体(luteinizing hotmone recep-tor,LHR)的一种剪接体(F类)在黄体4个不同发育时期(前,中,后,末)的相对表达差异进行了分析。同时将Re-al-time PCR中获得的数据用Realplex软件进行相对基因表达差异分析,应用SPSS软件对结果进行统计学分析。结果表明,LHR的F类剪接体在牛黄体发育的4个时期中,中期表达量是前期的0.535倍,后期表达量达到最高,是前期的5.65倍,末期显著下降是前期的0.011 6倍,各个时期LHR的F类剪接体表达量间存在极显著差异(P<0.01)。 A splice variant expression of luteinzing hormone receptor （LHR） in different stages of bovine corpus luteum was analyzed by real-time RT-PCR detection systems based on TaqMan-MGB probes. The results indicated that, the comparative quantities of the splice expression in different stages of bovine corpus luteum showed a marked difference. Compared with prophase, the metaphase expression is a marked 0. 535-fold, the latter stage is 5.65 times, the end stage cant decline 0. 011 6 times. LHR expression was significant differences among the four stages （P〈 0.01）.
作者 徐靖博 任久生 柳思源 李付娟 张阳阳 王帅 孙广杰 朱亚楠 高研 张嘉保
机构地区 吉林大学畜牧兽医学院 吉林大学实验动物中心
出处 《中国兽医学报》 CAS CSCD 北大核心 2012年第12期1893-1895,1900,共4页 Chinese Journal of Veterinary Science
基金 国家自然科学基金资助项目(30972100)
关键词 牛 黄体 LHR TAQMAN-MGB探针 bovine corpus luteum LHR Taqman-MGB probe
分类号 S823.8 [农业科学—畜牧学]
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参考文献12

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二级参考文献42

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共引文献5

中国兽医学报
2012年 第12期

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