丹皮酚对急性哮喘模型小鼠胸腺基质淋巴细胞生成素表达的影响

Effects of Paeonol on the expression of TSLP in a murine models of asthma

目的观察丹皮酚对急性哮喘小鼠模型气道炎症以及对胸腺基质淋巴细胞生成素(TSLP)表达的影响。方法 BALB/c小鼠48只随机分成正常对照组、哮喘模型组、丹皮酚组、布地奈德组,每组12只。卵蛋白致敏,气道激发,丹皮酚组给予丹皮酚100 mg/kg灌胃,1次/d,末次激发24 h后,检测各组小鼠气道反应性。HE染色观察气道炎症变化;采用ELISA观察支气管肺泡灌洗液(BALF)中IL-4、IL-13和血清总IgE的表达,real-time PCR观察TSLP的表达,Western-blot观察TSLP蛋白表达。结果哮喘组小鼠气道炎症和气道高反应性明显加重,丹皮酚能够显著抑制慢性哮喘小鼠模型的气道炎症和气道高反应性,哮喘BALF中Th2细胞因子IL-4、IL-13和血清总IgE含量显著降低。丹皮酚治疗后哮喘小鼠肺组织高表达的TSLP的mRNA和蛋白水平显著降低。结论丹皮酚能够抑制哮喘小鼠模型的气道炎症和气道高反应性,其机制可能通过抑制TSLP的的表达而实现。

Purpose To observe the effects of Paeonol on the expression of TSLP in a murine model of asthma. Methods 48 BALB/c mice were randomly divided into 4 groups：control group,asthma group, Paeonol group and Budesonide group. The mice were challenged with aerosolized 1% ovalbumin（OVA）. Mice in the Paeonol group were intragastrically administered with Paeonol, （100 mg/kg） daily. 24 hours after the last OVA challenge. Pulmonary functions were measureed to evaluate the resistance of expiration. The sections were stained with either hematoxylin or eosin to assess the inflammatory cell infiltrates. The levels of IL-4 and IL-13 in bronchoalveolar lavage fluid （BALF） , and total IgE levels in serum were meas- ured by ELISA. The mRNA of TSLP were measured by real time PCR. The protein expression of TSLP was determined by Western blot. Results Airway inflammation was increased in the OVA group. Treat- ment with paeonol, markedly inhibited the airway inflammation and airway hyperresponsiveness （AHR）. Treatment with paeonol significantly decreased the levels of IL-4 and IL-13 in BLAF and total IgE levels in serum. Also, Paeonol obviously attenuated the mRNA and protein expression of TSLP. Conclusion This study demonstrated that Paeonol could suppress the progression of airway inflammation and AHR in a murine model of asthma. The effect might be partly due to inhibition of the expression of TSLP.