吴茱萸次碱对实验动物急性心肌缺血损伤的保护作用

Protective effect of rutaecarpine on acute myocardial ischemia in animals

目的探讨吴茱萸次碱(Rut)对实验动物急性心肌缺血损伤的保护作用。方法采用垂体后叶素及异丙肾上腺素致小鼠急性心肌缺血模型,检测血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)的活性。进一步采用大鼠冠状动脉结扎模型,检测血流动力学指标及血清LDH、CK、超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量。结果与模型组相比,Rut组能降低垂体后叶素及异丙肾上腺素所致小鼠血清LDH及CK的升高(P<0.05)。在大鼠冠状动脉结扎实验中,与模型组相比,Rut组能改善结扎大鼠血流动力学指标的变化,血清中LDH、CK的酶活力及MDA的含量下降,SOD活力升高(P<0.05)。结论 Rut对实验动物急性心肌缺血有一定的保护作用。

Purpose To investigate the protective effect of rutaecarpine on acute myocardial ischemia in animals. Methods Myocardial ischemia models were induced by pituitrin and isoproterenol in mice to test the level of serum lactate dehydrogenase （LDH） , and creatine kinase （CK） activities. Left anterior ） descending coronary artery ligation was used in rats to test the hemodynamic parameters and the level of serum LDH, CK, malodialdehyed （MDA） , and superoxide dismutase （SOD） activities. Results Rutae- carpine treatment group compared with model group ： the levels of LDH and CK in serum were lowered ob- viously （P 〈 0.05） in mice. It also improved the cardiac function, and lowered LDH, CK, MDA while increased SOD（P 〈0.05） activity in rats. Conclusion Rutaecarpine has protective effect on experimental acute myocardial ischemia formation.