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乌苏里蝮蛇毒一种C-型凝集素相关蛋白的分离、纯化及活性测定 被引量：3

Separation,purification and vital determination of a C-type agglutinin coherent protein in Gloydius ussuriensis toxin
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摘要 目的从乌苏里蝮蛇蛇毒中分离纯化得到具有促凝血活性的C-型凝集素相关蛋白。方法利用IDA-Sepharose FF亲和色谱作为独立的蛋白分离纯化手段,并且结合Sephadex G-100,Sephadex G-50凝胶过滤色谱从乌苏里蝮蛇蛇毒中分离得到一个新的蛋白组分。结果该组分经还原和非还原SDS-PAGE电泳鉴定结果显示为均一的单一条带,即C-型凝集素相关蛋白,相对分子质量约为15.7 kD。结论经过一系列的分离和纯化,能够从乌苏里蝮蛇蛇毒中提取到C-型凝集素相关蛋白组分。 Purpose To purify a C-type agglutinin coherent protein in Gloudius ussuriensis toxin. Methods Firstly by using IDA-sepharose FF affinity chromatography and sephadex G-100,sephadex G-50 filtration chromatography a new protein from Gloydius ussuriensis toxin has been separated. Results The new protein was idientified by reduction and non-reduction SDS-PAGE electrophovesis. It showed a singu- lar zone and its molecular was 15.7 kD. In the protein biological activity determination,the results found that the protein had procoagulant activity directly and the activity had relevance to promoting its fibrinogen solidly. They should belong to C-type agglutinin. Conclusion By the separation and purification, a C-type agglutinin coherent protein in Gloudius ussuriensis toxin could be obtained.
作者 刘立 田刚 宋艳 孙德军
机构地区 白求恩医科大学制药厂 吉林大学再生医学科学研究所生物技术药物教研室
出处 《中国生化药物杂志》 CAS CSCD 北大核心 2012年第6期805-808,共4页 Chinese Journal of Biochemical Pharmaceutics
关键词 乌苏里蝮蛇蛇毒 C-型凝集素相关蛋白(CLRPs) 分离纯化 活性测定 Gloydius ussurien.sis C-type agglutinin separation and purification vital determination
分类号 TQ464.9 [化学工程—制药化工]
  • 相关文献

参考文献5

  • 1Morita T. Structures and functions of snake venom CLPs ( C-type lectin-like proteins)with anticoagulant-,procoagulant-, and plate- let-modulating activities [ J ]. Toxicon, 2005,45 ( $ ) : 1099-1114.
  • 2李文辉,张云.蛇毒C-型凝集素研究进展[J].Zoological Research,2003,24(2):151-160. 被引量：10
  • 3郭尧君.蛋白质电泳实验技术[M].2版.北京:科学出版社,2001:123-157.
  • 4Bradford M M. A rapid and sensitive method for quantitation of mi- crogram quantities of protein utilizing the principle of protein-dye- binding[ J ]. Anal B iochem, 1976,72 ( 2 ) :248-254.
  • 5Ouyang C,Teng C M. Fibrinogenolytic enzymes of trimeresurus mu- crosquamatus venom [J]. Biochim Biophys Acta, 1976,420 ( 2 ) : 298 -308.

二级参考文献44

  • 1Andrews RK,Kroll MH,Ward CM,Rose JW,Scarborough RM,Smith AI,Lopez JA,Berndt MC.1996.Binding of a novel 50-kilodalton alboaggregin from Trimeresurus albolabris and related viper venom proteins to the platelet membrane glycoprotein Ⅰb-Ⅸ-Ⅴ complex.Effect on platelet aggregation and glycoprotein Ⅰb-mediated platelet activation[J].Biochemistry,35:12629-12639.
  • 2Arocas V,Zingali RB,Guillin MC,Bon C,Jandrot-Perrus M.1996.Bothrojaracin:A potent two-site-directed thrombin inhibitor[J].Biochemistry,35:9083-9089.
  • 3Atoda E,Morita T.1989.A novel blood coagulation factor Ⅸ/factor Ⅹ-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (habu snake):Isolation and characterization[J].J.Biochem.,106:803-813.
  • 4Atoda H,Hyuga M,Morita T.1991.The primary structure of coagulation factor Ⅸ/Ⅹ binding protein isolated from the venom of Trimeresurus flavoviridis[J].J.Biol.Chem.,266:14903-14911.
  • 5Atoda H,Ishikawa M,Yoshihara E,Sekiya F,Morita T.1995.Blood coagulation factor Ⅸ-binding protein from the venom of Trimeresurus flavoviridis:Purification and characterization[J].J.Biochem.,118:965-973.
  • 6Atoda H,Ishikawa M,Mizuno H,Morita T.1998.Coagulation factor Ⅹ-binding protein from Deinagkistrodon acutus venom is a Gla domain-binding protein[J].Biochemistry,37:17361-17370.
  • 7Blockmans D,Deckmyn H,Vermylen J.1995.Platelet activation[J].Blood Reviews,9:143-156.
  • 8Brinkhous KM,Read MS,Fricke WA.1983.Botrocetin (venom coagglutinin):Reaction with a broad spectrum of multimeric forms of factor Ⅷ macromolecular complex[J].Proc.Natl.Acad.Sci.,80:1463-1466.
  • 9Chen YL,Tsai IH.1996.Functional and sequence characterization of coagulation factor Ⅸ/factor Ⅹ-binding protein from the venom of Echis carinatus leucogaster[J].Biochemistry,35:5264-5271.
  • 10Clemetson JM,Polgar J,Magnenat E,Wells TNC,Clemetson KJ.1999.The platelet collagen receptor glycoprotein Ⅵ is a member of the immunoglobulin superfamily closely related to FcαR and the natural killer receptors[J].J.Biol.Chem.,274:29019-29024.

共引文献9

同被引文献39

  • 1Alex N Zelensky,Jill E Gready.REVIEW ARTICLE: The C-type lectin-like domain superfamily. The ESP Journal . 2005
  • 2Franziska T. Arlinghaus,Johannes A. Eble.??C-type lectin-like proteins from snake venoms(J)Toxicon . 2012 (4)
  • 3R. Doley,R. M. Kini.??Protein complexes in snake venom(J)Cellular and Molecular Life Sciences . 2009 (17)
  • 4Takashi Morita.??Structures and functions of snake venom CLPs (C-type lectin-like proteins) with anticoagulant-, procoagulant-, and platelet-modulating activities(J)Toxicon . 2005 (8)
  • 5Tomohisa Ogawa,Takahito Chijiwa,Naoko Oda-Ueda,Motonori Ohno.??Molecular diversity and accelerated evolution of C-type lectin-like proteins from snake venom(J)Toxicon . 2004 (1)
  • 6Katsunori Horii,Daiju Okuda,Takashi Morita,Hiroshi Mizuno.??Crystal Structure of EMS16 in Complex with the Integrin α2-I Domain(J)Journal of Molecular Biology . 2004 (2)
  • 7Kurt Drickamer.??C-type lectin-like domains(J)Current Opinion in Structural Biology . 1999 (5)
  • 8Drickamer K.Two distinct classes of carbohydrate-recognition domains in animal lectins. Journal of Biological Chemistry . 1988
  • 9Morita Takashi.Structure-function relationships of C-type lectin-related proteins. Pathophysiology of haemostasis and thrombosis . 2006
  • 10Sakurai Y,Fujimura Y,Kokubo T,Imamura K,Kawasaki T,Handa M,Suzuki M,Matsui T,Titani K,Yoshioka A.The cDNA cloning and molecular characterization of a snake venom platelet glycoprotein Ib-binding protein, mamushigin, from Agkistrodon halys blomhoffii venom. Thrombosis and Haemostasis . 1998

引证文献3

中国生化药物杂志
2012年 第6期

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