不同pH与蛋白含量的胶原海绵植入缺损骨组织部位的疗效及安全性考察

The Curative Effect and Safety of Collagen Sponge with Different pH and Content of Protein Implanted into Orthopedic Bone Defect

手术构建家兔桡骨3mm完全缺损的骨折模型,在骨缺损部位植入不同pH及蛋白含量的胶原海绵,各组动物于术后进行体温测量、血常规、血清抗体、X光片及组织病理检查,考察胶原海绵的疗效和安全性。结果发现胶原海绵具有良好的安全性,但在血常规、血清抗体水平、病理等方面,随着pH及胶原含量变化会呈现轻微的改变,表明不同pH胶原海绵对组织无明显的安全性影响,低pH条件在一定程度上可促进缺损组织愈合,但会影响血常规及血清抗体水平;而低含量组海绵的安全性更佳。

This paper presents a 3mm fracture model of radial bone of rabbits using operation method. The bone de fect was treated with collagen sponge with different pH and different content. After the operation, the body temper ature, blood routine measurement, serum antibody, Xray examination and histological observation in each group were examined to evaluate and study the curative effect and safety of collagen sponge. Collagen sponge had a good re sult of safety, but there was slightly change in blood routine, serum antibody, and histological observation, etc, with the pH changing and different content of collagen. The results showed that there was no obvious influence of safety to tissues after treatment of the collagen sponge at different pH implanted into bone defect. Collagen sponge at lower pH could promote the healing of bone defect partly, while the safety of collagen sponge with lower content was better.