IL-3体外造血支持效应的初步研究

The preliminary study on the supportive effect of IL-3 in vitro on hematopoiesis

目的 :探讨IL 3体外促进脐血造血细胞增殖分化的效应。方法 :连续 2次Ficoll Hypaque分离 ,初步纯化脐血造血细胞 ,将其单个核细胞接种于经γ射线预处理的成人骨髓基质细胞层上 ,加入适量IL 3,并设不加IL 3的对照组 ,持续培养 6w ,定期检测非粘附细胞和集落形成细胞 (CFC)。结果 :IL 3组 3～ 6w非粘附细胞数和CFC产率显著高于对照组 ,分别为对照组的 2 .7～4.5倍和 1.6～ 2 .3倍。结论 :IL

Objective:To elucidate the enhanced effect of IL3 on the proliferation and differentiation of cord blood hematopoietic cells in vitro.Methods:To preliminarily purify cord blood hematopoietic cells by continuiously double FicollHypaque method.The adult marrow stromal layer was establishied by static liquid culture,then irradiated with γray.The mononuclear cells(MNCs) were cultured in stromalcontact system with or without IL3 for six weeks.The nonadherent cells and CFCs were counted and measured at different time point.Results:The experimental group with IL3 was remarkable greater increase than control group in terms of the number of nonadherent cells(2.7～4.5 fold increase)and the production of CFCs(1.6～2.3 fold increase).Conclusion:IL3 enhanced the proliferation and differentiation of positive hematopoietic cells from cord blood in the stromasupported culture.