血管内皮细胞生长因子与乙型肝炎病变的关系

Expression of vascular endothelial growth factor in liver tissues of hepatitis B

目的研究血管内皮细胞生长因子（ｖａｓｃｕｌａｒ ｅｎｄｏｔｈｅｌｉａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ， ＶＥＧＦ）与乙型肝炎（乙肝）分级（Ｇ）和分期（Ｓ）的关系。方法２５０例乙肝患者肝活检标本用ＶＥＧＦ单克隆抗体进行免疫组织化学（Ｓ－Ｐ法）染色。结果ＶＥＧＦ在肝组织表达有胞浆型、膜窦型及窦内皮细胞型；ＶＥＧＦ主要由肝细胞分泌，其次在肝窦内皮细胞、贮脂细胞、成肌纤维细胞及单核细胞也示阳性。无明显病变的血管内皮细胞及肝细胞ＶＥＧＦ染色阴性。提示随分级增高ＶＥＧＦ表达增强，其间差异有极显著意义（ｘ~２＝９０．４３，Ｐ＜０．０１）；而Ｓ低分值者ＶＥＧＦ多有阳性表达，分值较高者多为强阳性表达，两者差异明显（ｘ~２＝１１５７４，Ｐ＜０．０１）。结论ＶＥＧＦ表达与乙肝Ｇ和Ｓ相关。

Objective To study the relationship between vascular endothelial growth factor (VEGF) and grading (G) and staging (SS) of hepatitis B (HB). Methods Immunohistochemical staining was performed with VEGF monoclonal antibody in 250 biopsies of HB. Results The types of VEGF expression in liver tissues were of plasma type, sinusoidal membrane type and sinusoidal endothelial cell type. VEGF was mainly secreted by hepatocytes, and was also positively expressed in liver sinusoidal endothelial cells, fat-storing cells, myofibroblast and monomorphological nuclear cells. And it was negative in vascular endothelial cells and hepatocytes which had not significant damage, suggesting that the expression of VEGF was enhanced with the increase of G (x^2=90.43, P<0.01). VEGF was positively expressed on those whose scores of S were lower, while strongly positively expressed on those whose scores of S were higher. There was also a significant difference between them (x^2= 115.74, p<0.01). Conclusion The expressions of VEGF are related to G and S of HB.