肠源性内毒素血症在硫代乙酰胺所致肝损伤发病中的作用

Effects of intestinal endotoxemia on pathogenesis of liver injury induced by thioacetamide

目的探讨肠源性内毒素血症在硫代乙酰胺（ｔｈｉｏａｃｅｔａｍｉｄｅ，ＴＡＡ）所致肝损伤模型发病机制中的地位和作用。方法选用雄性Ｗｉｓｔａｒ大鼠２６只，随机分为４组，即正常组（Ｎ）及结肠切除（Ｃ）对照组； ＴＡＡ损伤组（Ｔ）和结肠切除＋ＴＡＡ组（Ｃ＋Ｔ）。采用生化检测法测定血浆内毒素含量和ＡＬＴ活性。结果单纯给予ＴＡＡ组，血浆内毒素水平和ＡＬＴ活性显著高于其它三个组；而结肠切除十ＴＡＡ组的血浆内毒素水平与结肠切除对照组和正常对照组相比无明显升高，ＡＬＴ活性结肠切除组＋ＴＡＡ组和正常对照组比较明显升高，但比ＴＡＡ组却明显减低。 Ｔ组与 Ｃ＋Ｔ组血浆内毒素水平与 ＡＬＴ活性变化之间呈正相关关系（ｒ＝ ０．９８５， Ｐ＜ ０．０１）。结论 ＴＡＡ所导致的内毒素血症是肠源性内毒素血症；ＴＡＡ本身有直接致肝细胞损伤的作用，但其所形成的肠源性内毒素血症造成的肝损伤更为严重。

Objective To study the effect of intestinal endotoxemia on pathogenesis of liver injury induced by thioacetamide (TAA). Methods Twenty-six male Wistar rats were divided into 4 groups, namely: normally control group (N), colectomy control group (C), colectomy+TAA group (C+T), TAA group (T). The changes in plasma biochemistry were measured. Results Plasma endotoxin levels were significantly higher in TAA group than normally control group. Plasma endotoxin levels in colectomy+TAA group were close to that of normally control group. Plasma ALT activity in colectomy+TAA group increased significantly compared with that of normal control group, but markedly lower than that in TAA group. Plasma endotoxin levels were positively correlated to ALT activity in T and C+T groups (r=0.985, p<0.01). Conclusion TAA itself has a deleterious effect to hepatic cells. It can also induce intestinal endotoxemia in which the liver injury is more inclined to occur and severe.