生姜根际一株纤维素降解共生菌的分析

Analysis of a Commensal Strain Producing Cellulase from Zingiber officinale Rhizosphere

对一株从生姜根际筛选到的纤维素降解活性较高的菌株SP1进行了初步分析。通过PCR方法获得菌株SP1的16S rDNA序列,长度为1 406 bp。使用摇床发酵法对菌株SP1发酵产纤维素酶的特征进行了初步分析。结果表明,该菌株产酶的最适pH为7,适宜温度为40℃,发酵40 h能达到产酶高峰。试验首次分析了生姜根际纤维素降解共生菌的情况,并为开发新型天然纤维素酶提供了资料。

A Zingiber officinale rhizosphere strain SP1 with higher cellulose-decomposing activity was preliminarily analyzed. With PCR, a 1 406 bp 16S rDNA fragment of SP.I was amplified. The method of table concentrator fermentation was used to preliminarily analyze the characteristics of SP1 cellulose. The result shows, pH 7 was the best acidity, 37℃ was the best temperature, and 40 h of fermentation could reach the peak of enzyme activity. Analyze ginger commensal cellulose-decomposing strain for the first time and provided data to develop new nature cellulase.