5-FU联合人参黄芪复方对人胃癌MGC-803细胞生物学行为的影响

Effects of 5-FU Combined Compound Ginseng and Astragalus on Biological Behavior of Human Gastric Cancer MGC803 Cells

目的探讨中药人参黄芪复方联合5-氟尿嘧啶(5-FU),在体外对人胃癌MGC-803细胞的增殖、克隆、凋亡、迁移等生物学行为的影响。方法采用MTT方法检测细胞的增殖抑制率;并计算其中效浓度;流式细胞仪检测观察细胞的周期及凋亡;Giemsa染色检测细胞的克隆形成;细胞划痕实验检测药物对细胞迁移的抑制作用。结果人参黄芪复方和5-FU均能抑制人胃癌MGC-803细胞生长,并随着药物浓度的增加而增强,5-FU联合人参黄芪复方对细胞生长的抑制率明显高于单用人参黄芪复方或5-FU组(P<0.05),并随着浓度的增加而增强;人参黄芪复方和5-FU均能诱导胃癌细胞凋亡、抑制细胞克隆形成、阻滞细胞于G0/G1期、并抑制细胞迁移,而两药联用时细胞凋亡率、G0/G1期细胞均明显高于两药单用(P<0.05),并阻滞细胞于G0/G1期;人参黄芪复方与5-FU联合应用时细胞克隆形成、细胞迁移面积均明显低于两药单用(P<0.05),且联合用药的中效浓度小于两药单用时的中效浓度之和。结论人参黄芪复方和5-FU联合应用与两药单用比较,能更好的抑制人胃癌MGC-803细胞增殖、抑制细胞克隆形成、促进细胞凋亡、阻滞细胞期于G0/G1期,并抑制细胞迁移。

Objective To observe the in vitro effects of 5-fluorouracil （5-FU） combined Compound Ginseng and Astragalus （CGA） on the biological behaviors such as the proliferation, the cloning, apoptosis and migra- tion of human gastric cancer MGC-803 cells. Methods The cell proliferation inhibition rate was detected by MTT assay, and the median effective concentrations of different drugs used alone/combination were calculated. The cell cycle and apoptosis were observed by flow cytometry. The formation of the cell colony was detected by Giemsa staining. The drugs＇ inhibition on cell migration was detected by cell scratch experiment. Results CGA and 5-FU both could inhibit the growth of the human gastric cancer cell line MGC-803 cells, and their effects were enhanced along with increased drug concentrations. Compared with CGA or 5-FU alone, CGA ＋5-FU got higher cell growth inhibition rate （ P 〈0. 05）, and the effects were enhanced along with increased concentrations. CGA and 5-FU both could induce the apoptosis of MGC-803 cells, inhibit the formation of cell cloning, block cells at G0/G1 phase, and inhibit the cell migration. Compared with CGA or 5-FU alone, CGA ＋5-FU got higher apoptosis rate of MGC- 803 cells, and more cells blocked at Go/G1 phase （P〈0. 05）. Besides, the MGC-803 cells were inhibited at G0/G1 phase. Compared with CGA or 5-FU alone, CGA ＋5-FU obviously lowered formation of cell cloning and area of cell migration （P〈0. 05）. The median effective concentration of CGA ＋5-FU was less than the sum of the median effective concentration of CGA and 5-FU. Conclusion Compared with CGA or 5-FU alone, CGA ＋5-FU could better inhibit the cell growth of human gastric cancer MGC- 803 cells, suppress the formation of cell cloning, induce cell apoptosis, block the cell cycle at G0/G1 phase, and inhibit the cell migration.