摘要
目的观察通心络对缺血性脑卒中大鼠脑组织微血管新生及血流灌注状态的影响。方法利用开颅结扎法阻断大鼠一侧大脑中动脉制作局灶性脑缺血模型(MCAO),筛选后随机分为假手术组、模型组、通心络大、中、小剂量组和尼莫地平组,给药14天后,免疫组化方法测定MCAO大鼠微血管密度(CD31)表达,激光扫描共聚焦显微镜观察脑组织微血管形态及血流灌注量。结果通心络大、中剂量组,尼莫地平组CD31蛋白阳性分布密集且着色显著,均强于模型组;通心络大、中剂量组,尼莫地平组均能够促进脑缺血皮层区微血管的血流灌注量(P<0.01,P<0.05),其中通心络大剂量组效果显著(P<0.01)。结论通心络显著增加缺血侧脑组织微血管密度,促进脑缺血后微血管新生,增加缺血脑组织的血流灌注量,发挥一定的血流代偿作用。
Objective To observe the effects of Tongxinluo （TXL） on angiogenesis and the volume of blood perfusion in ischemic stroke rats. Methods The model of middle cerebral artery occlusion （MCAO） was established using craniotomy ligation of the middle cerebral artery on one side. After screening, the male SD rats were randomly divided into the sham-operation group, the model group, the large dose TXL group, the middle dose TXL group, the low dose TXL group, and the Nimodipine group. The expression of microvascular density （ MVD, CD31 ） of the MCAO rats was detected using immunohistochemical assay after 14 days of medication. The microvascular morphology and the volume of blood perfusion in the brain tissue were observed under laser scan- ning confocal microscope （LSCM）. Results The positive CD31 expression was intense with significant coloring in the large dose TXL group, the middle dose TXL group, and the Nimodipine group, better than that of the model group. The blood perfusion volume in the ischemic brain cortex could be promoted in the large dose TXL group, the middle dose TXL group, and the Nimodipine group （ P 〈 0.01, P 〈 0. 05）. The optimal effects were shown in the large dose TXL group （P〈0.01）. Conclusion TXL significantly increased the MVD of the ischemic brain tissue, promoted the post-ischemic angiogenesis, and increased the volume of blood perfusion of ischemic brain tissue, playing certain blood flow compensatory roles.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第12期1667-1670,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家重点基础研究发展计划(973计划)(No.2005CB523301
No.2012CB518606)
