粒细胞集落刺激因子及其受体在儿童神经母细胞瘤中的表达研究

Expression of granulocyte colony-stimulating factor and its receptor in childhood neuroblastoma

目的检测粒细胞集落刺激因子(G-CSF)及其受体(G-CSFR)在儿童神经母细胞瘤中的表达情况。方法收集2009年1月-2011年6月确诊的神经母细胞瘤患儿手术标本25例。应用免疫组织化学法检测肿瘤标本中G-CSF和G-CSFR的表达,分析G-CSF,G-CSFR的表达与患儿年龄、性别及肿瘤临床分期的相关性。结果神经母细胞瘤标本中,G-CSF和G-CSFR阳性表达率分别为68%、72%。其中,G-CSF与G-CSFR均呈阳性者12例(48%),均呈阴性者2例(8%);一致性检验显示,G-CSF与G-CSFR同时检测均为阳性的标本无一致性(Kappa=-0.0456,P=0.8187)。G-CSF阳性与G-CSFR阴性者5例(20%),G-CSF阴性与G-CSFR阳性者6例(24%)。G-CSF和G-CSFR的表达与性别、年龄及临床分期无显著相关性(P>0.05)。结论无论性别、年龄及临床分期,G-CSF和G-CSFR在儿童神经母细胞瘤中均高表达,对于G-CSFR阳性表达的神经母细胞瘤患者,外源性应用G-CSF应慎重考虑其对肿瘤的影响。

Objective To study the expression of granulocyte colony-stimulating factor （G-CSF） and its receptor （G-CSFR） in neuroblastoma of children. Methods Twenty-five specimens of neuroblastoma were collected in our department during 2009.1-2011.6. G-CSF and G-CSFR were determined by immunohistochemistry. The correlation between expressions of G-CSF and G-CSFR and age, gender and clinical stage were analyzed. Results The expression of G-CSF and G-CSFR in neuroblastoma specimens was 68%, 72% respectively. Both G-CSF and G-CSFRwere positive in 12（48%）specimens, and both G-CSF and G-CSFR were negative in 2 cases （8%）. But according to test of concordance, the positive expression of G-CSF and G-CSFR had no concordance in neuroblastoma specimens （Kappa=--0.0456, P=0.8187）. There were 5 cases （20%） showed positive-G- CSF but negative-G-CSFR, 6 cases（24%） with negative expression of G-CSF but positive-G-CSFR. No statistical correlation was found between the expression of G-CSF or G-CSFR and gender, age and clinical stage （P〉0.05）. Conclusions G-CSF and G-CSFR are highly expressed in children neuroblastoma, regardless of age, gender and clinical stage. For the G-CSFR-positive patients, we recommend the use of G-CSF, and its effect on tumor should be carefully observed.