摘要
采用报告基因分析的方法 ,构建SRY基因 5′旁侧具有启动效应的 5 44bp以内不同长度片段与荧光素酶报告基因载体PGL2 Enhancer的重组子 ,并通过脂质体转染技术导入Hela细胞 ,检测各重组子报告基因的瞬间表达 ,由此分析启动子不同模块对基因转录效率的影响。结果显示 :位于起始密码子ATG上游nt.- 35 3～ - 174之间的 179bp片段内含有某种抑制子元件 ,nt.- 112～ - 6 3之间的 49bp片段内含有某种增强子元件 ,nt.- 174～ - 112之间的 6 3bp片段中含有基因转录所必须的启动子序列。进一步定位这些顺式作用元件 ,并研究与之相互作用的反式作用因子 。
The luciferase systems were used to assay the promoter activity of SRY gene with clones of different parts of the 5′ flanking region within 544 bp which has basal promoter activity. The results were that the 179 bp region from nt. -353 to nt.-174 upstream of the first ATG included a silent element; the 49 bp region from nt.-112 to nt.-63 included an enhanced element and the 63 bp region from nt.-174 to nt.-112 included an essential promoter sequency for gene transcription. These results give some important clues to elucidate the expression and regulation mechanisms of SRY gene. [
出处
《湖南医科大学学报》
CSCD
2000年第3期227-230,共4页
Bulletin of Hunan Medical University
基金
国家自然科学基金资助!(39570390)
