成人急性白血病免疫表型特征分析及意义

The features and significance of immunophenotyping in adult acute leukemia

采用活细胞间接免疫荧光技术 ,使用常规 8种抗CD单抗 ,测定 12 0例成人急性白血病免疫表型。结果显示免疫分型与FAB形态学分型有较高的符合率 ,但亦存在CD抗原在淋系或髓系交叉或反向表达、甚或不表达的情况。急性粒细胞白血病 (AML)M3 型和急性淋巴细胞白血病 (ALL)L3 型以及B淋巴系和T淋巴系ALL在免疫表型上各有其特点。

Immunophenotypic assay was determined by using indirect immunofluorescent method in 120 adult patients with acute leukemia. Eight monoclonal CD antibodies consisting of antiCD2,CD7,CD10, CD19, CD13, CD33, CD14, and antiHLADR monoclonal antibodies were applied conventionally. The results were that immunophenotyping had high corresponding rate to FAB morphology typing in diagnosing acute leukemia. Also there were some patients who had cross or inverse CD marker expression or no specific CD antigen expression. There were some immunophenotypic features in AMLM 3, ALLL 3, and BLineage and TLineage acute lymphocytic leukemia respectively. Prognostic significance was observed in the patients with specific immunophenotypes, that is acute myeloid leukemia patients with lymphoid antigen (Ly+AML) and some cases with inverse immunophenotypes. [