日本血吸虫DNA疫苗pCDSj32不同免疫次数及末次免疫后不同攻击时间对小鼠保护性免疫力的观察

INVESTIGATION ON PROTECTIVE EFFECTS OF DNA VACCINE PCDSJ32 FORSCHISTOSOMA JAPONICA BY DIFFERENT INJECTION TIMES AND CHALLENGE TIME AFTER THE LAST INOCULATION IN MICE

目的 观察日本血吸虫 DNA疫苗 p CDSj32不同免疫次数及末次免疫后不同攻击时间对小鼠保护性免疫力的影响。方法 分别以 10 0 μg/ 10 0 μl的 p CDSj32经股四头肌注射免疫 BAL B/ c小鼠 ,并分为一次免疫组合三次免疫组。各组于末次免疫后 4wk或 8wk,每只小鼠用 40条日本血吸虫尾蚴攻击感染。 6 wk后剖杀小鼠 ,观察各组小鼠的减成虫率和肝组织减卵率。结果 各免疫组与对照组相比均产生较好的保护免疫效果 ,减成虫率为 35 .6 1%～ 44 .44 % ,肝组织减卵率为 39.6 4%～6 9.0 6 %。结论 DNA疫苗不同免疫次数及末次免疫后不同攻击时间对小鼠抗血吸虫尾蚴攻击感染效果有一定的影响。该疫苗免疫一次及免疫后

Aim Protective immunity of DNA vaccine pCDSj32 for Schistosoma japonica by different injection times and challenge time after the last inoculation in mice was investigated. Methods 100μg/100μl DNA vaccine pCDSj32 was injected into the quadriceps muscles of the BALB/c mice. The mice were divided into once immunized groups and three times immunized groups. Challenge infection was applied 4wk or 8wk after the last inoculation with 40 cercariae each mouse. The adult reduction rates and the EPG in the livers of mice were detected 6wk after challenge. Results Substantially protective immunity was obtained when compared the immunized groups with the control. The adult reduction rates were 35.61～44.44%,and the egg reduction rates in the livers were 39.64～69.06%.Conclusion The protective immunity could be influenced by different times of DNA vaccine inoculation and the challenge time after the last immunization. The best protective results could be obtained with inoculation once combined with the challenge 8wk after the immunization of DNA vaccine.