双氢青蒿素对卡氏肺孢子虫超微结构的影响

THE EFFECT OF DIHYDROARTEMINSININ ON PNEUMOCYSTIS CARINII OBSERVED BY ELECTRON MICROSCOPY

目的 观察双氢青蒿素对卡氏肺孢子虫超微结构的影响。方法 以醋酸可的松皮下注射 Wistar大鼠 6周 ,诱导建立肺孢子虫肺炎动物模型 ,用双氢青蒿素治疗大鼠 ,并用电镜观察大鼠肺切片中肺孢子虫超微结构。结果 双氢青蒿素可使肺孢子虫滋养体胞浆及包囊内出现空泡 ,线粒体肿胀、核膜破裂、内质网肿胀 ,囊内小体溶解破坏等超微结构改变。

Aim To investigate the ultrastructural changes of Pneumocystis carinii treated with dihydroarteminsinin.Methods Pneumocystis carinii pneumonia (PCP) of immuosuppressed experimental rats models were established by injection subcutaeously with cortisone acetate twice a week for 6 weeks,the PCP rats were administered orally with dihydroarteminsinin at dose of 60mg/kg once a day for 5 days.The ultructural changes of P.carinii treated with dihydroarteminsinin were investigated by transmission electronic microscope.Results It was found that the ultrastructural changes of P.carinii treaed with the arteminsin derive included formation of vacuoles in trophozoites and cysts or precysts,swelling of mitochondria and endoplasmic reticulum,rupture of nuclear membranes,rupture and dissdve of intracyst bodies.Conclusion:It suggested that dihydroarteminsinin could mainly destroy membrane system ultrastructures of P.carinii.