蜂胶对致癌物诱导金仓鼠舌黏膜反应的影响(英文)

Effects of propolis on lingual mucosa response of hamsters submitted to experimental carcinogenesis

目的:研究9,10-二甲基-1,2-苯并蒽(9,10-dimethyl-1,2-benzanthracene,DMBA)以及蜂胶乙醇提取物(ethanol extract of propolis,EPE)对金仓鼠舌黏膜组织反应的影响。方法:60只金仓鼠按实验周期被随机分为两大组,每组再被分为EPE组、蜂胶水提取物(aqueous propolis extract,APE)组和DMBA组(n=20)。测定实验鼠舌黏膜的临床和组织学改变,包括面积、容积的临床改变,鳞状上皮组织的结构和细胞学变化,并测量鳞状细胞癌组织学上的改变区域。结果:各组在第13周和第20周所测定的实验结果不存在显著差异。在第13周,EPE组出现1例单一鳞状细胞癌;在第20周,EPE组的鳞状细胞癌的发生率最高,但与其他组比较差异无统计学意义。结论:蜂胶乙醇提取物对舌黏膜组织的影响以及其致癌机制还有待研究。

OBJECTIVE： To assess the tissue reaction of the lingual mucosa in hamsters submitted to daily, alternating, topical applications of 9,10-dimethyl-1,2-benzanthracene （DMBA） and a commercial brand of an ethanol propolis extract （EPE）. METHODS. A total of 60 hamsters were divided into three groups with two experimental periods （13 and 20 weeks）. The lateral edge of the tongue was submitted to daily, alternating, topical applications of 0.5% DMBA and 30% EPE （EPE group, n=20）, 0.5% of DMBA and aqueous propolis extract （APE group, n=20） and 0.5% of DMBA and saline solution （DMBA group, n=20）. The occurrence of clinical and histological alterations was analyzed, along with the measurement of the area and volume of the clinical alterations, the determination of structural and cytological alterations of the squamous epithelial tissue with atypias and the measurement of the histological area of squamous cell carcinomas. RESULTS. There were no significant differences among groups regarding any of the variables analyzed in the two evaluation periods. At week 13, a single squamous cell carcinoma occurred in the EPE group. At week 20, the greatest occurrence of squamous cell carcinoma was also in the EPE group. CONCLUSION： The mechanism of EPE （30% alcohol content） affecting the onset of tissue reaction and the promotion of carcinogenesis has not been clarified yet.