不同丁香提取物对戊四唑诱导的小鼠癫癎发作的影响(英文)

Effects of different extracts of Eugenia caryophyllata on pentylenetetrazole-induced seizures in mice

目的:研究不同丁香提取物对戊四唑引发小鼠癫癎的作用。方法:实验小鼠被随机分为生理盐水对照组、不同剂量(50、100、250及500mg/kg)丁香水提取物组、不同剂量(50、100、250及500mg/kg)丁香乙醇提取物组、不同剂量(50、100、250及500mg/kg)丁香三氯甲烷提取物组。给予戊四唑前60min为各组小鼠注射药物。记录并比较各组小鼠阵发性强直性抽搐潜伏期(generalized tonic-clonic seizure,GTCS)、最小阵发性痉挛潜伏期(minimal clonic seizure,MCS)及死亡率。结果:低、中、高三种剂量的丁香水提取物能有效延长MSC和GTCS的潜伏期(P<0.05)。50、100和250mg/kg的丁香乙醇提取物显著增加了小鼠MCS潜伏时间(P<0.05)。与盐水治疗组相比,惊厥小鼠模型经50、100、250和500mg/kg丁香的乙醇提取物治疗后,其GTCS的潜伏期显著高于盐水治疗组,但丁香的三氯甲烷提取物对惊厥小鼠的GTCS和MCS潜伏期没有显著的影响。结论:丁香乙醇和水提取物能抑制由戊四唑诱发的小鼠惊厥,但其确切的作用机制和有效的抗惊厥活性物质还有待进一步的研究。

OBJECTIVE： To investigate the possible anticonvulsant effect of different extracts of Eugenia caryophyllata （clove） on pentylenetetrazole （PTZ）-induced seizures in mice. METHODS： The animals were divided into saline, 50, 100, 250 and 500 mg/kg of aqueous extract, 50, 100, 250 and 500 mg/kg of ethanolic extract, and 50, 100, 250 and 500 mg/kg of chloroformic extract of clove groups. The extracts or saline were injected 60 min before each PTZ injection. Latency to the first minimal clonic seizure （MCS） and generalized tonic-clonic seizure （GTCS） and the percent of mortality were recorded. RESULTS： Aqueous extract of clove at doses of 50, 100, 250 and 500 mg/kg significantly extended the MCS and GTCS latency （P〈0. 05）. The MCS latency in mice treated with 50, 100 and 250 mg/kg of the ethanolic extract was significantly increased （P〈0. 05）. The GTCS latency in mice treated with 50, 100, 250 and 500 mg/kg of ethanolic extract was significantly higher than that of the saline-treated group （P〈0. 05）. There were no significant differences in MCS and GTCS latency between mice treated with different c%oroformic extract of clove or saline. CONCLUSION： The aqueous and ethanolic extracts of clove could inhibit the PTZ-induced convulsion, and this plant has the potential to be used as a new therapeutic agent for control of seizures. The exact mechanisms and the active compounds that are responsible for the anticonvulsive effect need to be clarified in future studies.