百草枯中毒小鼠肺组织转化生长因子β和缺氧诱导因子1α的表达

Expression of transforming growth factor-β and hypoxia inducible factor-1α in lung tissues of mice with paraquat poisoning

目的探讨百草枯中毒小鼠肺组织转化生长因子β(TGF-β)和缺氧诱导因子1α(HIF-1α)的表达变化。方法将30只雄性C57BL/6J小鼠随机分为对照组(n=10)和百草枯组(n=20)。百草枯组腹腔注射百草枯20 mg/kg,对照组腹腔注射等体积生理盐水。在染毒后第7天处死10只百草枯组小鼠,第28天处死其余小鼠。通过苏木精-伊红(HE)染色和Masson染色进行肺组织病理学观察;免疫组织化学法检测肺组织TGF-β和HIF-1α蛋白的表达。采用Pearson相关分析法分析TGF-β蛋白与HIF-1α蛋白表达的相关性。结果 HE染色和Masson染色结果均显示:在染毒后第7天,百草枯组可见肺纤维化表现;染毒后第28天,肺纤维化程度加重。染毒后第7天百草枯组小鼠肺组织TGF-β蛋白的表达明显高于对照组,染毒后第28天TGF-β蛋白的表达高于染毒后第7天,差异均有统计学意义(P<0.05)。染毒后第7天和第28天,百草枯组小鼠肺组织HIF-1α蛋白的表达均明显高于对照组(P<0.05);染毒后第28天的HIF-1α蛋白的表达高于第7天,但差异无统计学意义(P>0.05)。在染毒后第7天和第28天,百草枯组TGF-β蛋白与HIF-1α蛋白的表达均无相关性(r=0.295,P=0.630;r=0.218,P=0.725)。结论百草枯中毒可上调小鼠肺组织的TGF-β和HIF-1α蛋白,TGF-β和HIF-1α可能通过不同途径参与了肺纤维化的形成。

Objective To investigate the expression of transforming growth factor-β （TGF-β） and hypoxia inducible factor- lc~ （HIF-lct） in mice with paraquat poisoning. Methods Thirty male C57BL/6J mice were randomly divided into control group （n = 10） and paraquat group （n =20）. Paraquat （20 mg/kg） was intraperitoneally administered to mice in paraquat group, while the same volume of normal saline was intraperitoneally administered to those in control group. Ten mice in paraquat group were sacrificed on the 7th day after treatment with paraquat, and the rest of mice were killed on the 28th day. The pathological examinations of lung tissues were performed with HE staining and Masson staining, and the expression of TGF-β and HIF-1 α protein in lung tissues was detected by immunohistochemistry. The correlation of expression of TGF-β protein with expression of HIF-lα protein was explored by Pearson correlation analysis. Results HE staining and Masson staining indicated that there were presentations of lung fibrosis in paraquat group on the 7th day, and the severity of lung fibrosis increased on the 28th day. The expression of TGF-13 protein in lung tissues in paraquat group was significantly higher than that in control group on the 7th day （P 〈 0.05）, and the expression of TGF-13 protein in lung tissues on the 28th day was significantly higher than that on the 7th day in paraquat group （P 〈 0.05）. The expression of HIF-lα protein in lung tissues in paraquat group was significantly higher than that in control group on the 7th day and 28th day （P 〈 0.05）. The expression of HIF-lot protein in lung tissues on the 28th day was higher than that on the 7th day in paraquat group, while there was no significant difference between them （P 〉 0.05）. There was no correlation of expression of TGF-β protein with expression of I-IIF-lα protein on the 7th day and 28th day in paraquat group （ r = 0. 295, P = 0. 630; r = 0. 218, P = 0. 725）. Conclusion Paraquat poisoning can up-regulate TGF-β and HIF-lα protein in lung tissues of mice, and TGF-β