全反式维甲酸诱导前列腺癌细胞系DU-145凋亡的分子机理

Moleular mechanism of the apoptosis of prostate cancer cell line DU-145 cells induced by all trans retinoic acid

目的 克隆全反式维甲酸诱导前列腺癌细胞系 DU- 1 45细胞产生凋亡的相关基因。探讨维甲酸诱导前列腺癌细胞产生凋亡的分子机理。方法 应用全反式维甲酸诱导前列腺癌细胞系DU- 1 45细胞凋亡 ,采用基于 PCR的改良消减杂交技术克隆与凋亡相关的基因。结果 在前列腺癌 DU- 1 45细胞凋亡过程中 ,有大量肿瘤坏死因子 (TNF)的表达 ,同时 ,克隆出 c- erb B- 2基因。结论 TNF及 c- erb B- 2基因在由全反式维甲酸诱导的前列腺癌细胞系 DU- 1

Objective To Clone the apoptosis related genes induced by all trans retinoic acid(ATRA) from prostate cancer cell line DU 145 cells, and to investigate the moleular mechanism of the apoptosis of prostate cancer cells which was induced by ATRA.Methods The gene was cloned by improved PCR based subtractive hybridization from apoptotic prostate cancer cell line DU 145 cells induced by ATRA.Results During the apoptosis of DU 145 cells,a great amounts of TNF genes expressed, and c erb B 2 gene cloned.Conclusion TNF gene and c erb B 2 gene had an important roles during the apoptosis of DU 145 cells.