载超顺磁性氧化铁和阿霉素多功能高分子微泡的制备和体外显影实验研究

Preparation of polymer microbubbles encapsulated iron oxide nanopraticles and doxorubicin and characterization in vitro

目的制备一种载超顺磁性氧化铁和化疗药物阿霉素的多功能高分子微泡（MPMBs），探讨其体外超声和磁共振显像效果。方法采用双乳化法和冷冻干燥法制备MPMBs和普通高分子微泡（PMBs），检测前者的一般性质及其包封率和载药量，观察其体外释药特性以及超声和磁共振的显像效果。结果MPMBs形态规则，粒径分布较均匀，包封率为（60．20±2．69）％，载药量为（6．02±0．27）％；体外释药实验证明超声能促进MPMBs中阿霉素的释放；体外超声显影实验显示MPMBs较PMBs显影效果好，体外磁共振显影实验证实MPMBs具备增强磁共振显像的功能。结论成功制备出的MPMBs是一种具备治疗功能以及超声、磁共振双模式显影效果的多功能造影剂。

Objective To prepare a kind of superparamagnetic iron oxide （SPIO） and doxorubicin loaded multifunctional polymer microhubbles （MPMBs）, and to explore its potential application as an ultrasound（US）/magnetic resonance （MR） imaging contrast agent in vitro. Methods The MPMBs and normal polymer microhubbles （PMBs） were made by double emulsion and freeze-drying methods. The physical property,drug encapsulation efficiency and the drug-loading efficiency of MPMBs were determined, and the release property and US/MR imaging enhancement of MPMBs were observed. Results The MPMBs had a regular shape and narrow size distribution. The drug encapsulation efficiency was （60.20 ± 2.69） % ,and the drug-loading efficiency was （6.02±0.27）%. The in vitro release experiment showed that ultrasound can promote the release of doxorubicin in MPMBs. US imaging in vitro showed that the enhancement of MPMBs was better than PMBs, and MR imaging in vitro conformed that MPMBs could well enhance MR imaging. Conclusions The MPMBs is a multifunctional contrast agent with the treatment function as well as US/MR dual-mode imaging enhancement effect.