HBsAg阳性母亲新生儿树突状细胞数量变化的影响因素

Influence factors of quantitative changes of dendritic cells in neonate born to HBsAg positive mother

目的探讨HBsAg阳性母亲新生儿树突状细胞（DC）数量变化的影响因素。方法收集2011年7月至2012年3月在太原市第三人民医院妇产科分娩的HBsAg阳性孕妇及其新生儿60对。流式细胞术测定新生儿DC亚群数量，化学发光免疫试验检测母亲及新生儿出生24h内、主被动免疫前的外周血HBV血清学标志物，荧光定量PCR检测HBVDNA。两组样本比较采用两独立样本秩和检验中的Mann-Whitney检验和t检验，相关分析采用Spearman秩相关分析和卡方检验。结果60名新生儿中，5名为HBsAg阳性、HBVDNA阴性；60例HBsAg阳性孕妇中，HBeAg阳性21例，HBVDNA阳性29例。HBV宫内感染组新生JL#b周血髓样DC（mDC）、浆细胞样DC（pDC）与非感染组比较，差异无统计学意义（Z=-0．535，Z=-0．027；均P〉0．05）。母亲HBeAg阳性与新生儿HBeAg阳性密切相关（Pearson列联系数为0．928，P〈0．01）。HBeAg阳性和HBeAg阴性母亲所生新生儿外周血mDC分别为0．60±0．57和0．87±0．58（z=-2．085，P〈0．05）。HBVDNA阳性母亲所生新生儿外周血mDC、pDC与HBVDNA阴性者比较，差异无统计学意义（均P〉0．05）；母亲外周血HBVDNA〉1X107拷贝／mL与≤1×103拷贝／mL比较时，其新生儿外周血pDC明显下降（0．30±0．18比0．64±0．55，t=-2．996，P=0．005）。结论母亲HBeAg阳性可能使新生儿mDC数量下降，而HBVDNA〉1×107拷贝／mL时，可能会使新生儿pDC数量减少。

Objective To investigate the influence factors of quantitative changes of dendritic cells （DC） in neonate born to HBsAg positive mother. Methods Sixty HBsAg positive mothers and their newborns were enrolled from the Third People＇s Hospital of Taiyuan from July 2011 to March 2012. The serum hepatitis B virus （HBV） markers and HBV DNA in mothers and newborns before vaccination were determined by chemiluminescence immunoassay （CLIA） and fluorescence quantitative polymerase chain reaction （PCR）. The circulating frequencies of DC subgets were determined in the newborns by flow cytometry （FCM）. The comparison of data was done by Mann-Whitney test and t test. The correlation analysis was done by Spearman rank correlation analysis and chi square test. Results Among 60 newborns, 5 were HBsAg positive and HBV DNA negative. Among 60 HBsAgpositive mothers, 21 were HBeAg positive and 29 were HBV DNA positive. There was no significant quantitative difference of neonatal myeloid dendritic cells （mDC） and plasmacytoid dendritic cells （pDC） between intrauterine infection group and intrauterine non-infection group （Z=-0. 535, P = 0.59 and Z=-0. 027, P=0. 98, respectively）. However, motherrs HBeAg positive status was closely related with neonatal HBeAg positive status （Pearson contingency coefficient was 0. 928, P〈0.01）. The frequencies of mDC in newborns born to HBeAg positive mothers were significantly lower than those born to HBeAg negative mothers （0.60±0.57 vs 0.87±0.58 Z=-2. 085, P〈0.05）. However, there was no significant quantitative differences of mDC and pDC between newborns born to HBV DNA positive mothers and born to negative mothers （Z=-1. 272, P=0.20 and Z= -0. 806, P= 0.42, respectively）. The frequencies of pDC were significantly lower in newborns born to mothers with HBV DNA〉1 × 107 copy/mL compared to newborns born to HBV DNA negative mothers （0.30 ±0.18 vs 0.64±0. 55; t=-2. 996, P=0. 005）. Conclusions HBeAg positive status of mothers may reduce neonatal frequencies of mDC. Neonatal frequencies of pDC may be reduced when the mothers＇ HBV DNA loads are more than 1 × 107 copy/mL.